Mouse MAdCAM-1 Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF993U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MAdCAM-1
Gln22-Thr365, Predicted
Accession # NP_038619
Gln22-Thr365, Predicted
Accession # NP_038619
Specificity
Detects mouse MAdCAM-1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) MAdCAM-1, recombinant mouse (rm) ICAM-1, rmICAM-2, rmICAM-5, rmVCAM-1, and rhICAM-3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse MAdCAM-1 Alexa Fluor® 350-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: MAdCAM-1
Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule family member. In addition to Ig domains, it contains a mucin-like domain and a membrane proximal domain with similarity to IgA. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyer’s patches. It has also been found to be expressed on sinus-lining cells of the spleen. The integrin, alpha4 beta7, has been shown to function as the MAdCAM-1 receptor. The Ig domains of MAdCAM-1 have been found to be critical to alpha4 beta7 binding. The mucin domain has been shown to have activity in L-Selectin binding. MAdCAM-1 expression has been demonstrated to be up-regulated by TNF-alpha and IL-1 beta. MAdCAM-1 appears to play a role in inflammatory bowel disease (IBD) as its expression is highly up-regulated in IBD and most likely serves to recruit alpha4 beta7-expressing lymphocytes to the region. In vivo studies involving nonobese diabetic (NOD) mice have also suggested that MAdCAM-1/ alpha4 beta7 interaction plays a role in diabetes development in this model. Mouse MAdCAM-1 is a 405 amino acid (aa) residue protein with a 21 aa signal sequence, a 344 aa extracellular domain, a 20 aa transmembrane domain and a 20 aa cytoplasmic domain.
Long Name
Mucosal Addressin Cell Adhesion Molecule 1
Alternate Names
MAdCAM1
Gene Symbol
MADCAM1
UniProt
Additional MAdCAM-1 Products
Product Specific Notices for Mouse MAdCAM-1 Alexa Fluor® 350-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
Loading...
Loading...
Loading...