Mouse Lipocalin-2/NGAL Antibody

Name: Mouse Lipocalin-2/NGAL Antibody
Brand: R&D Systems
SKU: MAB18571

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB18571

Citations (2)

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Species Reactivity:	Mouse
Immunogen:	NS0-derived recombinant mouse Lipocalin-2/NGAL Gln21-Asn200 Accession # P11672
Applications:	ELISA Capture
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2A Clone # 228421
Specificity:	Detects mouse Lipocalin-2/NGAL in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) Lipocalin-1, rhLipocalin-2 or recombinant rat Lipocalin-2 is observed.

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Applications for Mouse Lipocalin-2/NGAL Antibody

Mouse Lipocalin-2/NGAL Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse Lipocalin‑2/NGAL Biotinylated Antibody (Catalog # BAM1857)

Standard: Recombinant Mouse Lipocalin-2/NGAL Protein, CF (Catalog # 1857-LC)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Lipocalin-2/NGAL

Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3‑5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (K_D = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less K_Dvalues (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

Item	Value
References	Hraba-Renevey, s. et al. (1989) Oncogene. 4:601. Liu, Q. et al. (1993) Mol Reprod Dev. 46:507. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272. Devireddy, L.R. et al. (2001) Science 293:829. Yang, M.B. et al. (2002) Mol. Cell. 10:1045. Goetz, D.H. et al. (2002) Mol. Cell 10:1033. Flo, T.H. et al. (2004) Nature 432:917. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
Long Name	Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs	3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names	24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL, LCN2, Lipocalin-2, Lipocalin2, MSFI, NGAL, NGALlipocalin-2, Oncogene 24p3, Siderocalin, Uterocalin, lipocalin 2, lipocalin 2 (oncogene 24p3), migration-stimulating factor inhibitor, neutrophil gelatinase-associated lipocalin, p25

Citations for Mouse Lipocalin-2/NGAL Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse Lipocalin-2/NGAL Antibody include:

Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

J Yan et al. (2020), T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation Cell Rep, 31(13):107820.
PMID: 32610123
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

Cheng L et al. (2015), Lipocalin-2 promotes m1 macrophages polarization in a mouse cardiac ischaemia-reperfusion injury model. Scand J Immunol, 81(1):31-8.
PMID: 25359467

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Mouse Lipocalin-2/NGAL Antibody

R&D Systems, Inc. a Bio-Techne Brand

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