Mouse LIMPII/SR-B2 Lumenal Loop Antibody

Name: Mouse LIMPII/SR-B2 Lumenal Loop Antibody
Brand: R&D Systems
SKU: AF1888

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1888

Citations (3)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse LIMPII/SR‑B2 Lumenal Loop Arg27-Thr432 Accession # O35114
Applications:	CyTOF-ready, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse LIMPII/SR‑B2 Lumenal Loop in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human (rh) LIMPII is observed and less than 1% cross‑reactivity with rhCD36 and rhSR‑B1 is observed.

View Full Technical Details for Mouse LIMPII/SR-B2 Lumenal Loop Antibody

Applications for Mouse LIMPII/SR-B2 Lumenal Loop Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse LIMPII/SR-B2 Fc Chimera (Catalog # 1888-LM)
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	0.25 µg/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse LIMPII/SR-B2 Lumenal Loop Antibody

	Intracellular Staining by Flow Cytometry Detection of LIMPII/SR-B2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse LIMPII/SR-B2 Affinity-purified Polyclonal Antibody (Catalog # AF1888, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: LIMPII/SR-B2

LIMPII (Lysosomal Integral Membrane Protein II), also known as LPG85 (85 kDa lysosomal membrane sialoglycoprotein) and CD36 antigen-like 2 (CD36L2), is a major lysosomal membrane protein. It belongs to the scavenger receptor class B subfamily and is designated member 2 (SR-B2). Other mammalian members of this family include SR-B1 (alternatively known as Cla-1 and CD36L1), and SR-B3 (CD36) (1‑3). SR-B/CD36 family members are type III integral membrane proteins with an N‑ as well as a C-terminal cytoplasmic tail, and a large extracellular (or luminal in the case of LIMPII) loop containing similarly spaced cysteine residues and multiple glycosylation sites. The C-terminal cytoplasmic tail has a di-leucine-based motif that mediates effective lysosomal targeting. LIMPII is expressed on all tissues and cell types examined so far, including activated platelets. LIMPII binds thrombospondin-1, but the biological significance of this interaction is not known. LIMPII‑thrombospondin interaction may contribute to the pro-adhesive changes of activated platelets during coagulation and inflammation (1). Overexpression of LIMPII causes an enlargement of early and late endosomes, suggesting that LIMPII may play a role in lysosome/endosome biogenesis (4). Mice deficient in LIMPII are impaired in membrane transport processes, resulting in ureteric pelvic junction obstruction, deafness and peripheral neuropathy (5).

Item	Value
References	Crombie, R. and R. Silverstein (1998) J. Biol. Chem. 273:4855. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785. Eskelinen, E-L. et al. (2003) Trends in Cell Biol. 13:137. Kuronita, T. et al. (2002) J. Cell Sci. 115:4117. Gamp, A-C. et al. (2003) Human Mol. Genet. 12:631.
Long Name	Lysosomal Integral Membrane Protein II
Entrez Gene IDs	950 (Human); 12492 (Mouse)
Alternate Names	AMRF, CD36 antigen, CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II), CD36L2, CD36L2LIMP II, HLGP85, LGP85, LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2, LIMPII, LIMPIICD36 antigen-like 2, LPG85, Lysosome membrane protein II, SCARB2, SR-B2, SR-BII, SRB2, Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein, lysosome membrane protein 2, scavenger receptor class B, member 2

Citations for Mouse LIMPII/SR-B2 Lumenal Loop Antibody (3)

Showing 1 - 3 of 3 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Mouse LIMPII/SR-B2 Lumenal Loop Antibody include:

Species: Mouse
Sample Types: BALF
Applications: ELISA Detection

AM Collin et al. (2020), Lung immunoglobulin A immunity dysregulation in cystic fibrosis EBioMedicine, 60(0):102974.
PMID: 32927272
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Casado M et al. (2012), HSL-knockout mouse testis exhibits class B scavenger receptor upregulation and disrupted lipid raft microdomains. J Lipid Res, 53(12):2586-97.
PMID: 22988039
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Yamayoshi S et al. (2011), Identification of a Human SCARB2 Region That Is Important for Enterovirus 71 Binding and Infection. J. Virol., 85(10):4937-46.
PMID: 21389126

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Product Documents for Mouse LIMPII/SR-B2 Lumenal Loop Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Mouse LIMPII/SR-B2 Lumenal Loop Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Mouse LIMPII/SR-B2 Lumenal Loop Antibody

Scientific Data Examples for Mouse LIMPII/SR-B2 Lumenal Loop Antibody

Intracellular Staining by Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: LIMPII/SR-B2

Citations for Mouse LIMPII/SR-B2 Lumenal Loop Antibody (3)

Product Documents for Mouse LIMPII/SR-B2 Lumenal Loop Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected