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Mouse Laminin  alpha1 / beta1 Antibody

Catalog # MAB4656 | R&D Systems, Inc. a Bio-Techne Brand
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MAB4656
MAB4656-SP

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # AL-4

Product Summary for Mouse Laminin  alpha1 / beta1 Antibody

Immunogen

Purified fragment of chymotrypsin-digested mouse EHS tumor-derived Laminin-1

Specificity

Detects the Hep-1 heparin binding domain of mouse Laminin A/alpha 1 subunit (1).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Laminin  alpha1 / beta1 Antibody

Laminin a1 antibody in Embryonic Mouse Decidua by Immunohistochemistry (IHC-Fr).

Laminin alpha1/ beta1 in Embryonic Mouse Decidua.

Laminin alpha1/ beta1 was detected in immersion fixed frozen sections of embryonic mouse decidua (E10.5) using 10 µg/mL Rat Anti-Mouse Laminin alpha1/ beta1 Monoclonal Antibody (Catalog # MAB4656) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Laminin a1 antibody in Mouse Kidney, Skin, Intestine, and Skeletal Muscle by Immunohistochemistry (IHC-Fr).

Laminin alpha1/ beta1 in Mouse Kidney, Skin, Intestine, and Skeletal Muscle.

Laminin alpha1/ beta1 was detected in perfusion fixed frozen sections of mouse kidney glomeruli (panel A), skin (panel B), intestine (panel C), and skeletal muscle (panel D) using Rat Anti-Mouse Laminin alpha1/ beta1 Monoclonal Antibody (Catalog # MAB4656) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse Laminin  alpha1 / beta1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse decidua (E10.5), and perfusion fixed frozen sections of mouse kidney glomeruli, skin, intestine, and skeletal muscle

Western Blot

Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349. This application was not tested by R&D Systems.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB4656 in the following applications:

Published Applications

Read 2 publications using MAB4656 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin alpha 1

Laminins are heterotrimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. Through interactions with integrins, dystroglycan, and other receptors, Laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes, and survival. Laminin-1 is comprised of alpha1, beta1, and gamma1 subunits.

References

  1. Skubitz, A.P. et al. (1988) J. Biol. Chem. 263:4861.
  2. Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349.

Alternate Names

LAMA1

Entrez Gene IDs

284217 (Human); 16772 (Mouse); 316758 (Rat)

Gene Symbol

LAMA1

Product Documents for Mouse Laminin  alpha1 / beta1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Laminin  alpha1 / beta1 Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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