Detects mouse L1CAM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM, rhNCAM-L1, rhOBCAM, recombinant mouse (rm) MAdCAM-1, or rmOCAM is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse L1CAM PE-conjugated Antibody

Detection of L1CAM in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse L1CAM PE-conjugated Monoclonal Antibody (Catalog # FAB5674P, filled histogram) or isotype control antibody (IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of L1CAM in Neuro-2A cells by Flow Cytometry.

Neuro-2A cells were stained with Rat Anti-Mouse L1CAM PE-conjugated Monoclonal Antibody (Catalog # FAB5674P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse L1CAM PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse splenocytes and Neuro-2A mouse neuroblastoma cells

Reviewed Applications

Read 1 review rated 4 using FAB5674P in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: L1CAM

L1CAM, also known as Neural Cell Adhesion Molecule L1 (NCAM-L1) and CD171, is a 200‑220 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, L1/Neurofascin/NgCAM family of molecules. L1CAM is expressed by neurons, especially by growing axons on their growth cones. Non-neuronal cells such as Schwann cells, astrocytes, epithelial cells, and cells of myelomonocytic and lymphoid origin also express L1CAM. Mature mouse L1CAM consists of a 1104 amino acid (aa) extracellular domain (ECD) with 6 Ig-like domains and 5 fibronectin type-III domains, a 23 aa transmembrane region, and a 114 aa cytoplasmic tail. Mouse L1CAM shares 88% aa sequence identity with human L1CAM. L1CAM is critical for neural development. Specifically, L1CAM plays a key role in neuronal cell migration, axon outgrowth, axon fasciculation, synaptogenesis, and myelination. L1CAM mediates homophilic cell-cell interaction but also binds heterophilically with Axonin-1, CD24, CD9, Neurocan and several Integrins. L1CAM can undergo membrane‑proximal cleavage by ADAM10 and ADAM17, leading to the release of the soluble ECD and the generation of a membrane‑bound stub. The soluble ECD can serve as a substrate for integrin-mediated cell adhesion, thereby stimulating cellular motility and cell migration. L1CAM also plays a role in the ontogeny of human tumors, and its expression is linked to poor prognosis. Proteolytic processing by ADAM10 and presenilin/ gamma-secretase is essential for "forward signaling" where an L1CAM intracellular domain translocates to the nucleus and participates in gene regulation. Defects in L1CAM are the cause of the neurological MASA/CRASH syndrome. In addition, uncleaved L1CAM can cluster with Integrin alpha5 either in-cis, or in-trans, inducing IL-1 beta expression and subsequent NF- kappaB activation. This is referred to as "reverse signaling".

Long Name

Cell Adhesion Molecule L1

Alternate Names

CAML1, CD171, HSAS, HSAS1, MASA, MIC5, NCAM-L1, S10, SPG1

Entrez Gene IDs

3897 (Human); 16728 (Mouse); 50687 (Rat)

Gene Symbol

L1CAM

Additional L1CAM Products

Product Documents for Mouse L1CAM PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse L1CAM PE-conjugated Antibody

For research use only

Citations
Reviews

Mouse L1CAM PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5674P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all L1CAM Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse L1CAM PE-conjugated Antibody

Detection of L1CAM in Mouse Splenocytes by Flow Cytometry.

Detection of L1CAM in Neuro-2A cells by Flow Cytometry.

Applications for Mouse L1CAM PE-conjugated Antibody

Flow Cytometry

Reviewed Applications

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: L1CAM

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

Additional L1CAM Products

Product Documents for Mouse L1CAM PE-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Mouse L1CAM PE-conjugated Antibody