Detects mouse Kell in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ECE-2, rhNeprilysin, or recombinant mouse Neprilysin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Applications for Mouse Kell Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Kell (Catalog # 1454-ZN)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kell

Kell, a type II membrane glycoprotein, is linked through a single disulfide bond to XK, a putative membrane transporter. The two proteins constitute the Kell blood group antigens (1). Kell is a zinc endopeptidase of the neprilysin (NEP) family which also includes endothelin converting enzymes (ECE-1 and ECE-2), PEX, XCE, DINE and several NEP-like proteins (2). It has been shown to cleave big endothelin-3 (ET-3) at Trp21-Ile22, yielding ET-3, and to a much lesser extent, big ET-1 and big ET-2 at Trp21-Val22, yielding ET-1 and ET-2 (3). Several different molecular defects cause the Kell null phenotype, which has no obvious clinical outcome (4).

References

Lee, S. et al. (2000) Transfus. Med. Rev. 14:93.
Turner, A.J. et al. (2001) BioEssays. 23:261.
Lee, S. et al. (1999) Blood 94:1440.
Lee, S. et al. (2001) J. Biol. Chem. 276:27281.

Long Name

Zinc protease blood group antigen

Alternate Names

CD238, KEL

Entrez Gene IDs

3792 (Human); 23925 (Mouse)

Gene Symbol

KEL

UniProt

Q9EQF2

Additional Kell Products

Product Documents for Mouse Kell Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Kell Antibody

For research use only

Citations
Reviews

Mouse Kell Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1454

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Kell Antibodies »

Product Specifications

Immunogen

Specificity