Mouse Integrin  alpha8 Biotinylated Antibody

Detection of Mouse Integrin alpha 8 by Immunocytochemistry/Immunofluorescence

RLCs selectively differentiate to intraglomerular mesangial cells during EC model.A. Representative confocal microscopy images for day 0 and day 7 of beta-gal and the EC markers ERG (upper panels) or CD31 (lower panels) co-stained kidney slices; B. Representative confocal microscopy images for day 0 and day 7 of beta-gal and the mesangial cell markers PDGF betaR (upper panels) or alpha8-integrin (lower panels) co-stained kidney slices; C. Representative confocal microscopy images for day 0 and day 7 of beta-gal/WT1 (podocyte marker) co-stained kidney slices. 4′,6-diamidino-2-phenylindole (DAPI) was used as a nuclear marker throughout. The channels for green ( beta-gal) and red (corresponding cell marker) fluorescent signals in the dashed squares on day 7 are separately shown in the small right panels. Scale bars correspond to 25 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29771991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Integrin alpha 8 by Flow Cytometry

Vascular development is limited in the reconstituted kidney organoids in vitro (A) Scheme for kidney organoid reconstitution and culture in vitro. Three types of kidney progenitors at E11.5 (NPs, SPs, and Hoxb7-GFP+ UBs) were aggregated overnight with or without ECs. The organoids were subsequently cultured at the air-liquid interface for 2–6 days (days 3–7). (B) Sorting of ECs, NP, and SPs. CD31+ and/or Flk1+ cells were sorted as ECs (left), and the cells in the CD31−/Flk1− fraction (left) were further sorted into Itga8+ NP and Pdgfra+ SP fractions (right). (C–F) Whole-mount staining of kidney organoids aggregated with ECs and cultured for the indicated days. GFP+ UB branching and CD31+ vasculature formation (C,D), as well as Nephrin+ glomerulus formation (E), were observed. No Cx40+ arterioles (F) were observed. (G) Section staining of glomeruli (nephrin). No CD31+ ECs were detected in glomeruli. (H–L) Whole-mount (H–K) or section (L) staining of kidney organoids aggregated without ECs and cultured for the indicated days. No apparent differences between the organoids with (C–G) and without (H–L) ECs were observed. Scale bars: 100 µm (C–F, H–K); 10 µm (G,L). Representative images of three organoids each with and without ECs from three independent experiments are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30718617), licensed under a CC-BY license. Not internally tested by R&D Systems.