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Mouse Integrin  alpha8 Biotinylated Antibody

Catalog # BAF4076 | R&D Systems, Inc. a Bio-Techne Brand
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BAF4076

Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse Integrin  alpha8 Biotinylated Antibody

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha8
Phe38-Phe1007
Accession # A2ARA8

Specificity

Detects mouse Integrin alpha8 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Integrin alpha5 and recombinant mouse (rm) Integrin alpha5 is observed, and less than 1% cross-reactivity with rmIntegrin alphaV is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Integrin  alpha8 Biotinylated Antibody

Integrin a8 antibody in 4T1 Mouse Cell Line by Immunocytochemistry (ICC).

Integrin alpha8 in 4T1 Mouse Cell Line.

Integrin a8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse Integrin a8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse Integrin  alpha8 by Western Blot.

Western blot shows lysates of Mouse Lung and Rat Lung. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Integrin  alpha8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4076) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. A specific band was detected for Integrin  alpha8 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Mouse Integrin alpha 8 by Immunocytochemistry/Immunofluorescence

Detection of Mouse Integrin alpha 8 by Immunocytochemistry/Immunofluorescence

Mesangial cells and not podocytes display considerable transient damage in the course of the EC model.Mice were analyzed at baseline (day 0), day 1, and day 7 following disease induction. A. Quantification of glomerular mesangial injury by alpha8-integrin (mesangial cell marker) staining of kidney slices. Data are presented as mean ± SEM, n = 5/5/10 for day 0 /day 1 /day 7, respectively. **p<0.01, ***p<0.001 by one-way ANOVA; B. Representative alpha8-integrin staining images in the course of the EC model. Scale bars correspond to 25 μm; C. Quantification of podocyte depletion and injury on kidney slices by WT1 and nephrin staining, respectively. Data are presented as mean ± SEM, n = 5/5/10 for day 0 /day 1 /day 7, respectively. *p<0.05, **p<0.01, ns- not significant by one-way ANOVA; D. Representative WT1/nephrin co-staining images in the course of the EC model. 4′,6-diamidino-2-phenylindole (DAPI) was used as a nuclear marker. Scale bars correspond to 25 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29771991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Integrin  alpha8 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample:  Immersion fixed 4T1 mouse breast cancer cell line

Western Blot

0.5 µg/mL
Sample: Mouse lung and rat lung
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using BAF4076 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 8

Integrin alpha 8 is a 170‑200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta1. alpha8 beta1 promotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse Integrin alpha8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 969 aa extracellular region (aa 38‑1006) and a 29 aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin  alpha8 shares 96% and 90% aa sequence identity with rat and human Integrin  alpha8 protein, respectively.

Alternate Names

ITGA8

Entrez Gene IDs

8516 (Human); 241226 (Mouse); 84381 (Rat)

Gene Symbol

ITGA8

UniProt

Product Documents for Mouse Integrin  alpha8 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Integrin  alpha8 Biotinylated Antibody

For research use only

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