Detects mouse Integrin alpha2/CD49b in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human Integrin alphaM is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha4, rmIntegrin alpha5, and rmIntegrin alphaE is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Integrin alpha2/CD49b Biotinylated Antibody

Integrin alpha2/CD49b in Mouse Splenocytes.

Integrin a2/CD49b was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Integrin a2/CD49b Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1740) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse Integrin alpha2/CD49b Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse DX5⁺ splenocytes

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Integrin alpha2/CD49b

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 2/CD49b

The Integrin family proteins are heterodimeric transmembrane receptors comprised of an alpha and a beta subunit. The Integrin alpha2 subunit is expressed in monocytes, platelets, B, T and NK cells. It forms a non-covalent heterodimer with the Integrin beta1 subunit. Integrin alpha2/ beta1 binds collagen and laminin and activates intracellular signaling pathways.

Alternate Names

CD49b, ITGA2, VLA-2 alpha

Entrez Gene IDs

3673 (Human); 16398 (Mouse)

Gene Symbol

ITGA2

UniProt

Q62469

Additional Integrin alpha 2/CD49b Products

Product Documents for Mouse Integrin alpha2/CD49b Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse Integrin alpha2/CD49b Biotinylated Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Integrin alpha2/CD49b Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1740

Key Product Details

Species Reactivity

Validated:

Cited:

Applications