Mouse IL-5 R alpha/CD125 Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF553AFP488

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AF553AFP488-100UG
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Goat IgG

View all IL-5 R alpha/CD125 Antibodies »

Product Specifications

Specificity

Detects mouse IL‑5 R alpha/CD125 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) IL‑5 R alpha is observed and less than 1% cross‑reactivity with recombinant mouse (rm) IL-4 R, rhIL-9 R, rhIL-13 R alpha1, and rhIL-13 R alpha2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Background: IL-5 R alpha/CD125

Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology (1‑3). Mature mouse IL‑5 R alpha consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 22 aa transmembrane segment, and a 54 aa cytoplasmic domain (4). Within the ECD, mouse IL‑5 R alpha shares 71% and 86% aa sequence identity with human and rat IL‑5 R alpha, respectively. Alternate splicing of mouse IL‑5 R alpha generates soluble secreted forms which function as IL‑5 antagonists (4, 5). The high affinity receptor for IL‑5 is a complex that consists of the ligand binding IL‑5 R alpha and the transmembrane common  beta chain ( betac/CD131) which is shared with the receptor complexes for IL‑3 and GM‑CSF (6). IL‑5 R alpha binds IL‑5 at low affinity and then associates with preformed betac oligomers to form the signaling‑competent receptor complex (7). IL‑5 stimulation of CD34+ hematopoietic progenitor cells induces the up‑regulation of transmembrane IL‑5 R alpha followed by eosinophilic differentiation and activation (8‑10). IL‑5 R alpha also promotes the differentiation of basophils and B cells (11, 12). Exposure of mature eosinophils to IL‑5 attenuates their IL‑5 responsiveness by inducing the down‑regulation of surface IL‑5 R alpha and increased production of soluble IL‑5 R alpha (13, 14). Elevated production of IL‑5 at sites of allergic inflammation induces eosinophilia and exacerbation of immune cell infiltration, tissue damage, and remodeling (2, 3).

References

  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Rothenberg, M.E. and S.P. Hogan (2005) Annu. Rev. Immunol. 24:147.
  3. Elsas, X.P. and M.I.G. Elsas (2007) Curr. Med. Chem. 14:1925.
  4. Takaki, S. et al. (1990) EMBO J. 9:4367.
  5. Cameron, L. et al. (2000) J. Immunol. 164:1538.
  6. Tavernier, J. et al. (1991) Cell 66:1175.
  7. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
  8. Tavernier, J. et al. (2000) Blood 95:1600.
  9. Clutterbuck, E.J. et al. (1989) Blood 73:1504.
  10. Lopez, A.F. et al. (1988) J. Exp. Med. 167:219.
  11. Denburg, J.A. et al. (1991) Blood 77:1462.
  12. Hasbold, J. et al. (2004) Nat. Immunol. 5:55.
  13. Gregory, B. et al. (2003) J. Immunol. 170:5359.
  14. Liu, L.Y. et al. (2002) J. Immunol. 169:6459.

Long Name

Interleukin 5 Receptor alpha

Alternate Names

CD125, IL-5Ra, IL5R alpha, IL5RA

Entrez Gene IDs

3568 (Human); 16192 (Mouse)

Gene Symbol

IL5RA

UniProt

P21183

Additional IL-5 R alpha/CD125 Products

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Certificate of Analysis

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Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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