Mouse IL-4R alpha Antibody

Name: Mouse IL-4R alpha Antibody
Brand: R&D Systems
SKU: MAB530
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB530

Citations (3)

(1)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse IL-4 R alpha Ile26-Arg233 Accession # P16382
Applications:	Western Blot
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2B Clone # 129801
Specificity:	Detects mouse IL-4 R alpha in direct ELISAs and Western blots. Shows less than 1% cross‑reactivity with recombinant human IL-4 R alpha.

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Applications for Mouse IL-4R alpha Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse IL‑4 R alpha Fc Chimera (Catalog # 530-MR)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-4R alpha

Interleukin 4 is a pleiotropic cytokine produced primarily by activated T cells, mast cells and basophils. The diverse biological effects of IL-4 on a variety of cell types are mediated by the binding of IL-4 to specific cell surface receptors. As is the case with many other cytokines, the functional high-affinity receptor for IL-4 is a complex consisting of a ligand binding subunit ( alpha chain) and a second subunit ( beta chain) that can modulate the ligand binding affinity of the receptor complex. It has been shown that in certain cell types, the gamma chain of the IL-2 receptor is a functional component ( beta chain) of the IL-4 receptor complex.

cDNA clones for the ligand binding chain (IL-4 R alpha ) of both the mouse and human high affinity IL-4 receptors have been isolated. The human or mouse IL-4 R alpha is an approximately 140 kDa transmembrane protein containing an extracellular domain, a transmembrane domain, and a large cytoplasmic domain that is essential for IL-4 signal transduction. In addition to the cDNA clone encoding the full-length transmembrane protein, a second cDNA clone that arises from alternate splicing and that encodes a soluble secreted form of IL-4 R alpha has been isolated from mouse cells, but not yet from human sources. A naturally-occurring soluble form of the IL-4 R alpha has also been identified in mouse biological fluids and murine cell culture supernatants.

Native or recombinant murine soluble IL-4 R alpha, as well as recombinant human soluble IL-4 R alpha, can bind IL-4 with the same affinity as the membrane bound IL-4 R alpha. Soluble IL-4 R alpha is a competitive inhibitor of IL-4 and has been shown to neutralize effectively many IL-4-mediated responses both in vivo and in vitro.

Item	Value
Long Name	Interleukin 4 Receptor alpha
Entrez Gene IDs	3566 (Human); 16190 (Mouse)
Alternate Names	CD124, CD124 antigen, IL-4 R alpha, IL-4 receptor subunit alpha, IL-4R alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4Ra, IL4R, IL4R alpha, IL4RACD124, interleukin 4 receptor, interleukin-4 receptor alpha chain, interleukin-4 receptor subunit alpha

Citations for Mouse IL-4R alpha Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-4R alpha Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

(2016), Multivalent Targeting Based Delivery of Therapeutic Peptide using AP1-ELP Carrier for Effective Cancer Therapy Theranostics, 6(12):2235-2249.
PMID: 27924160
Species: Human
Sample Types: Whole Cells
Applications: Western Blot

ES Kim et al. (2016), IL-4, a direct target of miR-340/429, is involved in radiation-induced aggressive tumor behavior in human carcinoma cells Oncotarget, 7(52):86836-86856.
PMID: 27895317
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Hiromura Y et al. (2007), IL-21 administration into the nostril alleviates murine allergic rhinitis. J. Immunol., 179(10):7157-65.
PMID: 17982108

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Mouse IL-4R alpha Antibody

Name: Anonymous

Application: WB

Sample Tested: Adipose tissue

Species: Mouse

Verified Customer | Posted 03/07/2022

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Product Documents for Mouse IL-4R alpha Antibody

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Mouse IL-4R alpha Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Mouse IL-4R alpha Antibody

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-4R alpha

Citations for Mouse IL-4R alpha Antibody (3)

Customer Reviews for Mouse IL-4R alpha Antibody (1)

Customer Images

Mouse IL-4R alpha Antibody

Product Documents for Mouse IL-4R alpha Antibody

Product Datasheets

COA

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SDS

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