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Mouse IL‑3 Antibody

Bio-Techne includes R&D Systems | Catalog # MAB403

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MAB403-100
MAB403-500
MAB403-SP

Key Product Details

Species Reactivity

Mouse

Applications

ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # MP28F8

Product Summary for Mouse IL‑3 Antibody

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse IL-3
Accession # P01586

Specificity

Detects mouse IL-3 in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant human (rh) IL‑3 or recombinant rat IL-3 is observed. Does not neutralize the biological activity of rhIL-3.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse IL‑3 Antibody

Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 Antibody.

Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 Antibody.

Recombinant Mouse IL-3 (Catalog # 403-ML) stimulates proliferation in the NFS60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-3 Monoclonal Antibody (Catalog # MAB403). The ND50 is typically 0.05-0.15 µg/mL.
Detection of Mouse IL-3 by Block/Neutralize

Detection of Mouse IL-3 by Block/Neutralize

MP-induced M2 skewing of SHIP-/- BM progenitors requires IL-4 and basophils but not IL-3.A) Western blots of MФs derived from BM for 6 days with M-CSF ± IL-3 (10 ng/mL) or MP (5%). B) Arginase activity of MФs from SHIP-/- BM derived for 6 days with 10 ng/mL M-CSF ± IL-3 (10 ng/mL) or MP (5%) with 2.5 μg/mL of either an irrelevant or IL-4-neutralizing Ab. Data (mean ± SD) are representative of 3 independent experiments performed in triplicate. * p < 0.05 compared to all other conditions. C) Western blot of SHIP-/- MФs from (B) probed for Ym1, using SHC as a loading control. Dashed lines indicate where irrelevant lanes have been cropped out. All lanes are part of the same time-exposed film on the same gel. D) Western blot of SHIP-/- basophil-depleted (DX5-) or basophils (DX5+) + DX5- BM derived with M-CSF ± MP (5%). E) Arginase activity of SHIP-/- MФs derived from BM for 6 days with M-CSF ± MP (5%) or IL-3 (10 ng/mL) ± neutralizing Ab to IL-3 (2.5 μg/mL) or IL-4 (2.5 μg/mL). F) Western blot of cells corresponding to panel (E). *** p < 0.001 compared to relevant control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27977740), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IL‑3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL‑3 (Catalog # 403-ML)

Neutralization

Measured by its ability to neutralize IL‑3-induced proliferation in the NFS60 mouse myeloid cell line. The Neutralization Dose (ND50) is typically 0.05 - 0.15 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑3.

Mouse IL-3 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse IL‑3 Biotinylated Antibody (Catalog # BAF403)
  • Standard: Recombinant Mouse IL-3 Protein (Catalog # 403-ML)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using MAB403 in the following applications:

Published Applications

Read 5 publications using MAB403 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated mouse mast cells, mouse keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and mouse IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on mouse cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of alpha and beta subunits. The IL-3 R alpha is a member of the cytokine receptor super family and binds IL-3 with low affinity. Two distinct beta subunits, AIC2A ( betaIL-3) and AIC2B ( betac) are present in mouse cells. betaIL-3 also binds IL-3 with low affinity and forms a high affinity receptor with the alpha subunit. The betac subunits does not bind any cytokine but forms functional high affinity receptors with the alpha subunit of the IL-3, IL-5 and GM-CSF receptors. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

References

  1. Yokota, T. et al. (1984) Proc. Natl. Acad. Sci. USA 81:1070.
  2. Fung, M.C. et al. (1984) Nature 307:233.
  3. Miyatake, S. et al. (1985) Proc. Natl. Acad. Sci. USA 82:316.

Long Name

Interleukin 3

Alternate Names

IL3, MCGF, MULTI-CSF

Entrez Gene IDs

3562 (Human); 16187 (Mouse); 24495 (Rat)

Gene Symbol

IL3

UniProt

Additional IL-3 Products

Product Documents for Mouse IL‑3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL‑3 Antibody

For research use only

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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