Murine p40 with D36K/W37K/T38K substitutions interacts with murine p19 but not with murine IL-12R beta1.A, structure of hIL-23 extracted from the structure of the IL-23:IL-23R complex structure (PDB 5mzv). A flexible linker sequence connecting p40 and p19 is indicated. B, Western blot analysis of secreted murine HIL-23, mp19 and mp40 variants from transfected CHO-K1 cells. The position of mp19 and mp40 variants is indicated by arrows. C, cellular proliferation of Ba/F3-gp130-mIL-12R beta1-mIL-23R cells. The cells were cultured for 3 days in the presence of 10 ng/ml HIL-6 or with the indicated cytokines (10% conditioned cell culture supernatant of transfected CHO-K1 cells). Parental Ba/F3-gp130 cells were used as controls. The results of one representative experiment of three are shown. Error bars represent S.D. for technical replicates. Statistical analysis used a one-way ANOVA, followed by Bonferroni correction (n = 3), ∗∗∗p ≤ 0.001. D, analysis of STAT3 and ERK1/2 activation. Ba/F3-gp130-mIL-12R beta1-mIL-23R cells were washed, starved, and stimulated with the indicated cytokines (10% conditioned cell culture supernatant of transfected CHO-K1 cells) for 30 min. Cellular lysates were prepared, and equal amounts of total protein (50 μg/lane) were loaded on SDS-PAA gels, followed by immunoblotting using specific antibodies for phospho-STAT3, STAT3, phospho-ERK1/2, and ERK1/2. Western blotting data show results of one representative experiment of two. E, co-IP of FLAG-tagged murine mp19 coexpressed with either mp40 or mp40D36K/W37K/T38K and full-length mIL-12R beta1 or mIL-23R. The position of mIL-23R and mIL-12R beta1 is indicated by arrows. One of two independent experiments is shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34637790), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IL-12 R beta1 Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL-12 R beta1 (Catalog # 1998-B1)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12 R beta 1

IL-12 R beta1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G‑CSF R family of cytokine receptors. IL-12 R beta1 is a common subunit of both the IL‑12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta1, respectively. It shares 16%‑21% aa sequence identity with the ECDs of mouse gp130, LIF R, G‑CSF R, and IL‑23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta1 with either IL-12 R beta2 or IL‑23 R (4‑6). IL‑12 R beta2 and IL‑23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8‑10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL‑23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta2 (13, 14).

References

Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
Chua, A.O. et al. (1995) J. Immunol. 155:4286.
Parham, C. et al. (2002) J. Immunol. 168:5699.
Wu, C. et al. (1997) J. Immunol. 159:1658.
Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
Airoldi, I. et al. (2000) J. Immunol. 165:6880.
Li, J. et al. (2003) J. Neurol. Sci. 215:95.
Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
Yen, D. et al. (2006) J. Clin. Invest. 116:1310.
Shimozato, O. et al. (2006) Immunology 117:22.
Russell, T.D. et al. (2003) J. Immunol. 171:6866.

Long Name

Interleukin 12 Receptor beta 1

Alternate Names

CD212, IL-12Rb1, IL12R beta 1, IL12RB1

Entrez Gene IDs

3594 (Human); 16161 (Mouse)

Gene Symbol

IL12RB1

UniProt

Q60837

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Product Specific Notices for Mouse IL-12 R beta1 Biotinylated Antibody

For research use only

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Mouse IL-12 R beta1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1998

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Scientific Data Images for Mouse IL-12 R beta1 Biotinylated Antibody

Detection of IL-12 R beta1 by Western Blot