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Mouse IGFBP‑6 Antibody

Catalog # MAB7761 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Key Product Details

Species Reactivity



ELISA Capture (Matched Antibody Pair), Western Blot



Antibody Source

Monoclonal Rat IgG2A Clone # 122312

Product Summary for Mouse IGFBP‑6 Antibody


Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-6
Accession # P47880


Detects mouse IGFBP-6 in ELISAs and Western blots. In Western blots, 10% cross-reactivity with recombinant human (rh) IGFBP-3 and rhIGFBP-5 is observed and no cross-reactivity with rhIGFBP-1, recombinant mouse (rm) IGFBP-1, rhIGFBP-2, rmIGFBP-2, rmIGFBP-3, rhIGFBP-4, rmIGFBP-5, or rhIGFBP-6 is observed.







Applications for Mouse IGFBP‑6 Antibody

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse IGFBP‑6 (Catalog # 776-B6) under non-reducing conditions only

Mouse IGFBP-6 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse IGFBP‑6 Biotinylated Antibody (Catalog # BAF776)
  • Standard: Recombinant Mouse IGFBP-6 Protein, CF (Catalog # 776-B6)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage


Protein A or G purified from hybridoma culture supernatant


Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-6

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Mouse IGFBP-6 cDNA encodes a 238 amino acid (aa) precursor protein with a putative 25 aa signal peptide that is processed to generate the 213 aa mature protein that is O-glycosylated. Mouse and human IGFBP-6 share 73% aa similarity. Mouse and rat IGFBP-6 share 94% aa similarity and the mouse IGFBP-6 has a 9 amino acid insertion compared to the rat homolog. IGFBP-6 is expressed in ovarian, testicular, muscle, heart and lung tissues in the adult mouse. IGFBP-6 was not detected in total RNA from a whole mouse embryo.


  1. Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57.
  2. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  3. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.

Long Name

Insulin-like Growth Factor Binding Protein 6

Alternate Names


Entrez Gene IDs

3489 (Human); 16012 (Mouse)

Gene Symbol



Product Documents for Mouse IGFBP‑6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IGFBP‑6 Antibody

For research use only

Reconstitution Calculator

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