Mouse IFN-beta Antibody

Name: Mouse IFN-beta Antibody
Brand: R&D Systems
SKU: 32400-1

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 32400-1

Citations (3)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	E. coli-derived recombinant mouse IFN-beta
Applications:	Block/Neutralize
Label:	Unconjugated
Source:	Polyclonal Rabbit Serum
Specificity:	Mouse IFN-beta

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Applications for Mouse IFN-beta Antibody

Neutralization

One neutralization unit is the amount of antiserum required to neutralize one unit of mouse interferon beta (Mu-IFN-beta ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) J. Virol. 37, 755-758] using L929 cells and EMCV as the challenge virus. In this antiviral assay for interferon, about 2.5 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined by use of a laboratory standard calibrated with respect to the international reference standard for Mu-IFN-beta provided by the National Institutes of Health [Gb02-902-511]. This material is prepared specifically for effective neutralization of Mu-IFN-beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Neat serum diluted in phosphate buffered saline (PBS)
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	After receipt, this product should be kept at -70˚C or below for retention of full activity.Thaw product vial by incubation in cold tap water until just thawed – the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath.

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Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Item	Value
Long Name	Interferon beta
Entrez Gene IDs	3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names	Fibroblast interferon, IFB, IFBIFNB, IFF, IFN-beta, IFNB, IFNB1, IFNbeta, MGC96956, interferon beta, interferon, beta 1, fibroblast

Citations for Mouse IFN-beta Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IFN-beta Antibody include:

Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)

BC Liu et al. (2018), Constitutive Interferon Maintains GBP Expression Required for Release of Bacterial Components Upstream of Pyroptosis and Anti-DNA Responses Cell Rep, 24(1):155-168.e5.
PMID: 29972777
Species: Mouse
Sample Types: Serum
Applications: ELISA Development

Sutherland DB et al. (2011), Evaluating vaccinia virus cytokine co-expression in TLR GKO mice. Immunol. Cell Biol., 89(0):706-15.
PMID: 21173782
Species: Mouse
Sample Types: Serum
Applications: ELISA Development

Joshi VD et al. (2006), A role for Stat1 in the regulation of lipopolysaccharide-induced interleukin-1beta expression. J. Interferon Cytokine Res., 26(10):739-47.
PMID: 17032168

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