Mouse IFN-alpha Antibody

Name: Mouse IFN-alpha Antibody
Brand: R&D Systems
SKU: 32100-1

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 32100-1

Citations (2)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	E. coli-derived recombinant mouse IFN-alpha A
Applications:	Block/Neutralize
Label:	Unconjugated
Source:	Polyclonal Rabbit Serum
Specificity:	Mouse IFN-alpha

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Applications for Mouse IFN-alpha Antibody

Neutralization

One Neutralization Unit (NU) is the amount of antiserum required to neutralize one unit of mouse interferon alpha (Mu-IFN-alpha ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay using L929 cells as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758] with the exception that EMCV was used as the challenge virus. In this antiviral assay for interferon, about 5 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Mu-IFN-alpha provided by the National Institutes of Health [Ga02-901-511). This material is prepared specifically for effective neutralization of Mu-IFN-alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	N/A
Formulation	Neat serum diluted in phosphate buffered saline (PBS)
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

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Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Item	Value
Long Name	Interferon alpha
Entrez Gene IDs	3440 (Human)
Alternate Names	IFN-alpha, IFN-alphaA, IFNA, IFNA2, IFNA2b, IFNalpha, INFA2, LeIF D, interferon alpha 2

Citations for Mouse IFN-alpha Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IFN-alpha Antibody include:

Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development

von Buttlar H et al. (2014), Identification of Toll-like receptor 9 as parapoxvirus ovis-sensing receptor in plasmacytoid dendritic cells. PLoS ONE, 9(8):e106188.
PMID: 25171368
Species: Mouse
Sample Types: Serum
Applications: ELISA Development

Joshi VD et al. (2006), A role for Stat1 in the regulation of lipopolysaccharide-induced interleukin-1beta expression. J. Interferon Cytokine Res., 26(10):739-47.
PMID: 17032168

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