Mouse ICOS Antibody

Name: Mouse ICOS Antibody
Brand: R&D Systems
SKU: AF168

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF168

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Species Reactivity:	Mouse
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ICOS Met1-Leu142 Accession # CAB71153
Applications:	Block/Neutralize, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse ICOS in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant mouse (rm) CTLA-4, rmCD28, and rmPD-1 is observed.

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Applications for Mouse ICOS Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS)
Blockade of Receptor-ligand Interaction	In a functional ELISA, 10-40 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Biotinylated Recombinant Human B7-H2 Fc Chimera to immobilized Recombinant Mouse ICOS (Catalog # 168-CS) coated at 1 µg/mL (100 µL/well). At 75 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: ICOS

Mouse ICOS (inducible co-stimulator), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Mouse ICOS is a homodimeric type I transmembrane protein consisting of 200 amino acids (aa) with a putative 20 aa signal sequence, a 122 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% aa similarity with CD 28 and CTLA-4. Mouse and human ICOS share approximately 72% aa identity. ICOS is expressed on most CD45RO⁺ cells. ICOS expression is upregulated within approximately 24‑48 hours of activation on T_h primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and T_h differentiation.

Item	Value
References	Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040. Yoshinaga, S.K. et al. (1999) Nature 402:827. Hutloff, A. et al. (1999) Nature 397:263. Aicher, A. et al. (2000) J. Immunol. 164:4689. Coyle, A.J. et al. (2000) Immunity 13:95. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
Long Name	Inducible Co-stimulator
Entrez Gene IDs	29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)
Alternate Names	AILIM, AILIMCD278, Activation-inducible lymphocyte immunomediatory molecule, CD278, CD278 antigen, CRP-1, CVID1, ICOS, MGC39850, inducible T-cell co-stimulator, inducible T-cell costimulator, inducible costimulator

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Mouse ICOS Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Mouse ICOS Antibody

Formulation, Preparation and Storage

Reconstitution Calculator

Background: ICOS

Citations for Mouse ICOS Antibody

Product Documents for Mouse ICOS Antibody

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