Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all ICAM-2/CD102 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ICAM‑2/CD102
Ser20-Gln222
Accession # P35330

Specificity

Detects mouse ICAM‑2/CD102 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human ICAM-2, recombinant mouse (rm) ICAM-1, rmMAdCAM-1, and rmICAM-5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse ICAM-2/CD102 Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse ovary

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse ICAM-2/CD102 Fc Chimera (Catalog # 774-M2)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-2/CD102

Intercellular Adhesion Molecule-2 (ICAM-2, also known as CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration. The mouse and human ICAM-2 proteins share 60% amino acid identity. Human LFA-1 binds equally well to human or mouse ICAM-2.

References

  1. Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
  2. Hayflick, J. et al. (1998) Immunologic Res. 17:313.
  3. Xu, H. (1992) J. Immunol. 149:2650.

Long Name

Intercellular Adhesion Molecule 2

Alternate Names

CD102, ICAM2

Entrez Gene IDs

3384 (Human); 15896 (Mouse)

Gene Symbol

ICAM2

UniProt

P35330

Additional ICAM-2/CD102 Products

Product Documents for Mouse ICAM-2/CD102 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse ICAM-2/CD102 Biotinylated Antibody

For research use only

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