Mouse GDF-3 Alexa Fluor® 532-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB953X
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 144728
Product Specifications
Immunogen
E. coli-derived recombinant mouse GDF-3
Ala253-Gly366
Accession # Q07104
Ala253-Gly366
Accession # Q07104
Specificity
Detects mouse GDF-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse GDF-1, -5, -6, -7, -8, or -9 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse GDF-3 Alexa Fluor® 532-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: GDF-3
GDF-3 (previously called Vgr-2) is a TGF-beta superfamily member belonging to the growth/differentiation factor family (1, 2). GDF-3 is expressed in undifferentiated embryonic stem (ES) cells, adipose tissue and the brain (2‑4). In ES cells, it maintains pluripotency and influences early cell fate decisions (5, 6). For example, frog embryos injected with GDF-3 develop a secondary dorsal axis and deletion of mouse GDF-3 can produce defects in the anterior visceral endoderm of the pre‑gastrulation embryo (5, 6). In adipocytes, GDF-3 is induced by a high fat diet and promotes adipogenesis (3). GDF-3 has been reported to oppose BMP’s functions and to have a nodal-like activity in early development (1). The 366 amino acid (aa) mouse GDF-3 contains a 22 aa signal sequence, a 230 aa propeptide and a 114 aa mature protein that contains one potential N-glycosylation site. Most of GDF-3 is present as the prepro form, while the mature GDF-3 is presumably the secreted, active form (1). The mature protein contains the cysteine-knot structure that is conserved throughout family members. Since it lacks the fourth cysteine, which is responsible for the formation of inter-molecular disulfide bond, GDF-3 may exist as a non-covalent homodimer. Mature mouse GDF-3 shares 90%, 83% and 83% aa identity with rat, human and canine GDF-3, respectively. Among family members, mature GDF-3 is most similar to mouse BMP-6 (45% aa identity) and Xenopus VG-1 (52% aa identity).
Long Name
Growth Differentiation Factor 3
Alternate Names
GDF3, Vgr-2
Gene Symbol
GDF3
UniProt
Additional GDF-3 Products
Product Specific Notices for Mouse GDF-3 Alexa Fluor® 532-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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