Mouse Galectin-4 Antibody

Name: Mouse Galectin-4 Antibody
Brand: R&D Systems
SKU: AF2128
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2128

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(3)

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Species Reactivity:	Mouse
Immunogen:	E. coli-derived recombinant mouse Galectin-4 Ala2-Ile326 Accession # Q8K419
Applications:	Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse Galectin-4 in direct ELISAs and Western blots.

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Applications for Mouse Galectin-4 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse Galectin‑4 (Catalog # 2128-GA)
Immunohistochemistry	3-15 µg/mL	Immersion fixed paraffin-embedded sections of Mouse Intestine

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse Galectin-4 Antibody

	Immunohistochemistry Detection of Galectin‑4 in Mouse Intestine. Galectin‑4 was detected in immersion fixed paraffin-embedded sections of Mouse Intestine using Goat Anti-Mouse Galectin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2128) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in intestinal epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Galectin-4

Galectins are a family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins share structural similarities in their carbohydrate recognition domains (CRD), forming three groups often termed prototype (one CRD), tandem-repeat (two CRDs) and chimeric (one CRD, unique N-terminus) (1, 2). All lack classical signal peptides, but are present and active both within and outside of the cell. Galectins are involved in cell adhesion, migration, survival, and apoptosis, and are often up- or down-regulated in cancer (1-3). Galectin-4 is a 36 kDa tandem-repeat galectin found throughout the gastrointestinal tract, but also present in well-differentiated breast and liver carcinomas (3, 4). Each CRD binds a different set of carbohydrate groups, including those found on erythrocyte blood group antigens (3, 5). CRD1 also binds cholesterol 3-sulfate and other sulfatides, which are concentrated within lipid raft membrane microdomains (6, 7). Endocytosed Galectin-4 is thought to play a role in forming the rafts, delivering them to the intestinal apical membrane, and stabilizing highly detergent-resistant "superrafts" (7-9). Human Galectin-4 shares 76%, 77%, 78%, and 80% amino acid (aa) identity with mouse, rat, bovine, and porcine Galectin-4, respectively, with the highest identity occurring within the CRDs. A potential splice variant begins at aa 132 and lacks most of the first CRD (10). Galectin-4 expression is concentrated within microvilli in the gastrointestinal epithelium, where it can interact with CD3 and bind activated T cells in the lamina propria during intestinal inflammation (11, 12). Either pro- or anti-inflammatory activity has been shown, depending on the mouse model used. Galectin-4 can also bind lung, spleen, and kidney macrophages, although its expression is normally low in these tissues (5).

Item	Value
References	Yang, R-Y. et al. (2008) Expert Rev. Mol. Med. 10:e17. Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679. Huflejt, M.E. and H. Leffler (2004) Glycoconj. J. 20:247. Recreche, H. et al. (1997) Eur. J. Biochem. 248:225. Markova, V. et al. (2006) Int. J. Mol. Med. 18:65. Ideo, H. et al. (2007) J. Biol. Chem. 282:21081. Delacour, D. et al. (2005) J. Cell Biol. 169:491. Braccia, A. et al. (2003) J. Biol. Chem. 278:15679. Stechly, L. et al. (2009) Traffic 10:438. Entrez accession # EAW56820. Hokama, A. et al. (2004) Immunity 20:681. Paclik, D. et al. (2008) PLoS ONE 3:e2629.
Entrez Gene IDs	3960 (Human); 16855 (Mouse)
Alternate Names	Antigen NY-CO-27, GAL4, Galectin-4, Galectin4, L-36 lactose-binding protein, L36LBP, LGALS4, Lactose-binding lectin 4, gal-4, galectin 4, lectin, galactoside-binding, soluble, 4

Citations for Mouse Galectin-4 Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse Galectin-4 Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Mycko M et al. (2014), Brain glycolipids suppress T helper cells and inhibit autoimmune demyelination. J Neurosci, 34(25):8646-58.
PMID: 24948818

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Customer Reviews for Mouse Galectin-4 Antibody (3)

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Mouse Galectin‑4 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Cancer Cells

Species: Human

Verified Customer | Posted 10/26/2015

This antibody is as good as the monoclonal antibody from R&D. Specificity: SpecificSensitivity: Reasonably sensitiveBuffer: PBSDilution: 1:1000
Mouse Galectin‑4 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Colon tissue

Species: Human

Verified Customer | Posted 10/26/2015

Very specific, Red is propidium iodide the nuclear stain. Specificity: SpecificSensitivity: Reasonably sensitiveBuffer: PBSDilution: 1:500
Mouse Galectin‑4 Antibody

Name: Anonymous

Application: 2D gel, protein modification

Sample Tested: Cell Lysates

Species: Human

Verified Customer | Posted 10/26/2015

Although there were mutliple bands, the one circled are galectin-4, the others are interactors isolated on a 2D gel. Using human cell lysates in 2D buffer. Specificity: SpecificSensitivity: SensitiveBuffer: BSADilution: 1:1000

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Mouse Galectin-4 Antibody

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Applications for Mouse Galectin-4 Antibody

Scientific Data Examples for Mouse Galectin-4 Antibody

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Galectin-4

Citations for Mouse Galectin-4 Antibody (1)

Customer Reviews for Mouse Galectin-4 Antibody (3)

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Mouse Galectin‑4 Antibody

Mouse Galectin‑4 Antibody

Mouse Galectin‑4 Antibody

Product Documents for Mouse Galectin-4 Antibody

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