Mouse FGF-23 Antibody

Name: Mouse FGF-23 Antibody
Brand: R&D Systems
SKU: MAB26291

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB26291

Citations (10)

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Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse FGF-23 Tyr25-Val251 (Arg179Gln) Accession # Q9EPC2
Applications:	Immunohistochemistry
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2A Clone # 283507
Specificity:	Detects mouse FGF-23 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 50% cross-reactivity with recombinant human (rh) FGF-23. In Western blots, this antibody does not cross-react with rhFGF-3, -4, -5, -7, -9, -10, -11, -12, -13, -16, -17, -18, -19, acidic, basic, rmFGF-8b, -8c, -15, or -21.

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Applications for Mouse FGF-23 Antibody

	Recommended Concentration	Sample
Immunohistochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse FGF-23 Antibody

	Immunohistochemistry FGF‑23 in Mouse Brain. FGF-23 was detected in perfusion fixed frozen sections of mouse brain (cortex) using Rat Anti-Mouse FGF-23 Monoclonal Antibody (Catalog # MAB26291) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: FGF-23

Fibroblast growth factor 23 (FGF-23) is a 30-32 kDa member of the FGF gene family. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members exist as highly diffusible molecules that is attributed to poor ECM/heparin sulfate binding (3-6). The cDNA for mouse FGF-23 predicts a 251 aa polypeptide that contains a 24 aa signal sequence and a 227 aa mature region (7). Mature mouse FGF-23 shows 72% aa identity to human FGF-23 (8). The FGF-19 subfamily shares an unusual receptor configuration. The standard model for FGF signaling requires an FGF:FGF R:heparin sulfate complex. Given FGF-23’s minimal association with heparin, a substitute termed ( alpha -) Klotho has evolved that serves the same function. Although FGF-23 binds to the widely expressed “c” isoforms of FGF R1 and 3 plus FGF R4, Klotho has a restricted distribution that limits FGF-23 activity (10-12). It should be noted that heparin-dependency has been reported for FGF-19 signaling, and this observation may extend to FGF-23 (13). The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism and all three are induced by a nuclear receptor heterodimer that includes the retinoid X receptor (14-16). FGF-23 is considered a phosphatonin; that is, a molecule that reduces circulating plasma phosphate. It is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone that induces hypercalcemia, and circulatory volume loading. Upon binding to FGF-23 receptors on renal proximal tubular epithelium, two basic changes are seen. First, the enzyme responsible for generating the active form of vitamin D is suppressed, resulting in decreased levels of bioactive vitamin D. Since vitamin D promotes intestinal phosphate absorption, plasma phosphate declines. Second, the transporters responsible for phosphate resorption on renal epithelium are down regulated, resulting in decreased uptake from urine and again a decline in blood phosphorus (17, 18).

Item	Value
References	Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107. Fukumoto, S. (2007) Endocr. J. Sep 14; [Epub ahead of print]. Huang, X. et al. (2006) Mol. Carcinog. 45:934. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417. Harmer, N.J. et al. (2004) Biochemistry 43:629. Yamashita, T. et al. (2000) Biochem. Biophys. Res. Commun. 277:494. Shimada, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6500. Kato, K. et al. (2006) J. Biol. Chem. 281:18370. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694. Urakawa, I. et al. (2006) Nature 444:770. Hurosu, H. et al. (2006) J. Biol. Chem. 281:6120. Wu, X. et al. (2007) J. Biol. Chem. 282:29069. Moore, D.D. (2007) Science 316:1436. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687. Razzaque, M.S. and B. Lanske (2007) J. Endocrinol. 194:1. Liu, S. et al. (2007) Curr. Opin. Nephrol. Hypertens. 16:329.
Long Name	Fibroblast Growth Factor 23
Entrez Gene IDs	8074 (Human); 64654 (Mouse)
Alternate Names	ADHR, FGF-23, FGF23, HPDR2, HYPF, PHPTC, Tumor-derived hypophosphatemia-inducing factor, fibroblast growth factor 23, phosphatonin, tumor-derived hypophosphatemia inducing factor

Citations for Mouse FGF-23 Antibody (10)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse FGF-23 Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

MM Hurley et al. (2022), FGF receptor inhibitor BGJ398 partially rescues osteoarthritis-like phenotype in older high molecular weight FGF2 transgenic mice via multiple mechanisms Scientific Reports, 12(1):15968.
PMID: 36153352
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

M Dixit et al. (2021), Induction of somatopause in adult mice compromises bone morphology and exacerbates bone loss during aging Aging Cell, 0(0):e13505.
PMID: 34811875
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

M Kawai et al. (2020), Lack of PTEN in osteocytes increases circulating phosphate concentrations by decreasing intact fibroblast growth factor 23 levels Scientific Reports, 10(1):21501.
PMID: 33299044
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot

H Sugiura et al. (2018), Fibroblast growth factor 23 is upregulated in the kidney in a chronic kidney disease rat model PLoS ONE, 13(3):e0191706.
PMID: 29518087
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

A Sakurai et al. (2017), Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone Biomed. Res., 38(4):257-267.
PMID: 28794403
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

N Kamiya et al. (2017), Targeted disruption of NF1 in osteocyte increases FGF23 and osteoid with osteomalacia-like bone phenotype J. Bone Miner. Res., 0(0).
PMID: 28425622
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

I-Ping Chen (2016), Dietary phosphate supplement does not rescue skeletal phenotype in a mouse model for craniometaphyseal dysplasia J Negat Results Biomed, 15(1):18.
PMID: 27784318
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Z Liu et al. (2016), Immunolocalization of osteocyte-derived molecules during bone fracture healing of mouse ribs Biomed Res, 37(2):141-51.
PMID: 27108883
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr

Spichtig D et al. (2014), Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease. Kidney Int, 85(6):1340-50.
PMID: 24402093
Species: Human
Sample Types: Whole Cells
Applications: ICC

Gore MO et al. (2008), Renal phosphate wasting due to tumor-induced osteomalacia: a frequently delayed diagnosis. Kidney Int., 0(0).
PMID: 18668025

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Mouse FGF-23 Antibody

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Applications for Mouse FGF-23 Antibody

Scientific Data Examples for Mouse FGF-23 Antibody

Immunohistochemistry

Formulation, Preparation and Storage

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Background: FGF-23

Citations for Mouse FGF-23 Antibody (10)

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