Mouse F4/80 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5580P

Catalog #
Availability
Size / Price
Qty
FAB5580P-100UG
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Conjugate
Catalog #
Unconjugated
MAB5580
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Alexa Fluor 350
FAB5580U
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Alexa Fluor 405
FAB5580V
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Alexa Fluor 488
FAB5580G
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Alexa Fluor 532
FAB5580X
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Alexa Fluor 594
FAB5580T
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Alexa Fluor 647
FAB5580R
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Alexa Fluor 700
FAB5580N
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Alexa Fluor 750
FAB5580S
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Alexa Fluor Plus 405
FAB5580AFP405
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Alexa Fluor Plus 488
FAB5580AFP488
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Alexa Fluor Plus 555
FAB5580AFP555
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Alexa Fluor Plus 594
FAB5580AFP594
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Alexa Fluor Plus 647
FAB5580AFP647
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Alexa Fluor Plus 680
FAB5580AFP680
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Allophycocyanin
FAB5580A
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Allophycocyanin/Cy7
FAB5580APCCY7
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Biotin
FAB5580B
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CoraFluor 1
FAB5580CL1
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DyLight 350
FAB5580D
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DyLight 405
FAB5580E
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DyLight 488
FAB5580K
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DyLight 550
FAB5580L
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DyLight 594
FAB5580M
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DyLight 650
FAB5580W
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DyLight 680
FAB5580Y
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DyLight 755
FAB5580Z
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FITC
FAB5580F
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HRP
FAB5580H
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Janelia Fluor 525
FAB5580JF525
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Janelia Fluor 549
FAB5580I
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Janelia Fluor 646
FAB5580J
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Janelia Fluor 669
FAB5580JF669
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mFluor Violet 450 SE
FAB5580MFV450
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mFluor Violet 500 SE
FAB5580MFV500
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mFluor Violet 610 SE
FAB5580MFV610
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PE/Atto594
FAB5580PEATT594
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PE/Cy5.5
FAB5580PECY55
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PE/Cy7
FAB5580PECY7
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 521204

View all F4/80 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549

Specificity

Detects mouse F4/80/EMR1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse F4/80 PE-conjugated Antibody

Detection of F4/80/EMR1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5580P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse F4/80 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: F4/80

F4/80, also known as EMR1 in humans, is a 160 kDa glycosylated protein that belongs to the EGF-TM7 family of adhesion molecules. It is expressed on splenic red pulp and bone marrow, macrophages, microglia, Langerhans cells, monocytes, eosinophils, and CD116+ dendritic cells in mouse, but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance, being necessary for the development of CD8+ T regs. It also serves as a marker for IL-1 beta+ proinflammatory macrophages.

Alternate Names

DD7A5-7, Gpf480, Ly71

Entrez Gene IDs

13733 (Mouse); 316137 (Rat)

Gene Symbol

ADGRE1

UniProt

Q61549

Additional F4/80 Products

Product Documents for Mouse F4/80 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse F4/80 PE-conjugated Antibody

For research use only

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