Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 338930

View all Epimorphin/Syntaxin 2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Epimorphin/Syntaxin 2
Met1-Arg189
Accession # Q00262

Specificity

Detects mouse Epimorphin/Syntaxin 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Epimorphin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Epimorphin/Syntaxin 2 Antibody

Epimorphin/Syntaxin 2 antibody in Embryonic Mouse Eye Cup by Immunohistochemistry (IHC-Fr).

Epimorphin/Syntaxin 2 in Embryonic Mouse Eye Cup.

Epimorphin/Syntaxin 2 was detected in immersion fixed frozen sections of embryonic mouse eye cup (13 d.p.c.) using 8 µg/mL Rat Anti-Mouse Epimorphin/Syntaxin 2 Monoclonal Antibody (Catalog # MAB2568) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Epimorphin/Syntaxin 2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse eye cup (13 d.p.c.)

Western Blot

1 µg/mL
Sample: Recombinant Mouse Epimorphin/Syntaxin 2

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Epimorphin/Syntaxin 2

Epimorphin, also known as syntaxin 2, is a member of the highly conserved syntaxin family. It is a type IV integral membrane protein with a C-terminal membrane anchor and an N-terminal functional domain. Epimorphin can assume membrane topology with the functional domain localized extracellularly or to the cytoplasmic face. Extracellular Epimorphin functions in morphogenesis while intracellular Epimorphin functions as a vesicle fusion protein. Mouse Epimorphin functional domain shares 97% and 91% amino acid sequence homology with rat and human Epimorphin, respectively.

Alternate Names

STX2, Syntaxin 2

Entrez Gene IDs

2054 (Human); 13852 (Mouse)

Gene Symbol

STX2

UniProt

Q00262

Additional Epimorphin/Syntaxin 2 Products

Product Documents for Mouse Epimorphin/Syntaxin 2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Epimorphin/Syntaxin 2 Antibody

For research use only

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