Mouse EpCAM/TROP‑1 PE‑conjugated Antibody

Catalog # FAB8998P | R&D Systems, Inc. a Bio-Techne Brand
Recombinant Monoclonal Antibody.
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FAB8998P-025
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rat IgG2A Clone # G8.8R

Product Summary for Mouse
EpCAM/TROP‑1 PE‑conjugated Antibody

Immunogen

TE-71 Thymic epithelial cell line

Specificity

Detects mouse EpCAM/TROP-1 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse EpCAM/TROP‑1 PE‑conjugated Antibody

Detection of EpCAM/TROP-1 antibody in 4T1 Mouse Cell Line antibody by Flow Cytometry.

Detection of EpCAM/TROP‑1 in 4T1 Mouse Cell Line by Flow Cytometry.

4T1 mouse breast cancer cell line was stained with Rat Anti-Mouse EpCAM/TROP-1 PE-conjugated Monoclonal Antibody (Catalog # FAB8998P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of EpCAM/TROP‑1 in mIMCD3 Mouse Cell Line by Flow Cytometry.

mIMCD3 mouse kidney cell line was stained with Rat Anti-Mouse EpCAM/TROP-1 PE-conjugated Monoclonal Antibody (Catalog # FAB8998P, filled histogram) or isotype control antibody (IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse EpCAM/TROP‑1 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: 4T1 mouse breast cancer cell line and mIMCD3 mouse kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using FAB8998P in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as ECD326 and TROP-1 is a 36-41 kDa member of the TROP family of meolcules (1, 2). It is a type I transmembrane glycoprotein that is found on embryonic stem cells and adult non-squamous epithelium such as (respiratory) psuedostratified, (mammary) cuboid, and (colon) simple columnar epithelium. In mouse, the molecule has also been reported on T cells, Langerhans cells and thymic epithelium. Mature mouse EpCAM is 292 amino acids (aa) in length. It possesses a 243 aa extracellular region that contains one thyroglobulin type I domain. Such domains are associated with cathepsin binding and inhibition (2). On the cell surface, EpCAM is reported to participate in TEM (Tetraspanin-enriched microdomain) formation in concert with CD44v6, CD9, ADAM10, Claudin-7 and TS8. It is also noted to form homodimers in-cis and homotetramers in-trans. Although this would suggest the EpCAM acts as an adhesion molecule, in effect it does not, instead acting as a disruptor of the E-Cadherin:cytoskeleton interaction, and thus promoting cell migration (1, 2). The extracellular domain (ECD) of EpCAM undergoes multiple cleavages, principally between Ser200 and Ala210 (3). Cleavage at the Arg80-Arg81 site does not generate a soluble fragment, but rather a disulfide-linked "heterodimer" (3). Cleavage after Gly264 in the juxtamembrane region is followed by secretase cleavage of the transmembrane fragment, generating an ICD that translocates to the nucleus (4). The ECD of mouse EpCAM shares 81% and 90% aa sequence identity with human and rat EpCAM, respectively.

References

  1. Schnell, U. et al. (2013) Biochim. Biophys. Acta 1828:1989.
  2. Martowicz, A. et al. (2015) Histol. Histopathol. Oct 23:11678 [ePub ahead of print].
  3. Schnell, U. et al. (2013) Biosci. Rep. 33:e00030.
  4. Hachmeister, M. et al. (2013) PLoS ONE 8:e71836.

Long Name

Epithelial Cell Adhesion Molecule

Alternate Names

17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1

Entrez Gene IDs

4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)

Gene Symbol

EPCAM

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse EpCAM/TROP‑1 PE‑conjugated Antibody

For research use only

Customer Reviews (1)

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  • Mouse EpCAM/TROP-1 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Tumor cell lyastes
    Species: Mouse
    Verified Customer | Posted 08/09/2023
    We employed a cluster of markers for the identification of mice fibroblasts. To conduct flow analysis, a final concentration of 10 µL/10^6 cells cells was applied. EPCAM and CD45 were both employed as negative staining markers for cancer-associated fibroblasts. The positive group was less frequent than anticipated, suggesting the need for a dilution control to accurately define positive staining.
    Mouse EpCAM/TROP‑1 PE‑conjugated Antibody FAB8998P

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FAQs for Mouse
EpCAM/TROP‑1 PE‑conjugated Antibody

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  • Q: I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?

    A: We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

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