Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all CXCL7/Thymus Chemokine-1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCL7/Thymus Chemokine‑1
Lys40-Tyr113
Accession # AAG36786

Specificity

Detects mouse CXCL7/Thymus Chemokine‑1 in ELISAs and Western blots. In sandwich ELISAs, less than 0.02% cross-reactivity with recombinant rat (rr) TCK-1, rrCINC-3, recombinant human GRO-beta, recombinant mouse (rm) BLC, rmCRG-2/IP-10, and rmMIG is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse CXCL7/Thymus Chemokine-1 Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CXCL7/Thymus Chemokine-1 (Catalog # 793-CK)

Mouse CXCL7/Thymus Chemokine-1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse CXCL7/Thymus Chemokine-1 Antibody (Catalog # MAB10911)
  • Standard: Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 48-109) (Catalog # 1091-CK)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL7/Thymus Chemokine-1

CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid residue precursor protein. Signal P prediction program analysis indicates that this precursor protein contains an amino terminal 39 amino acid residue signal peptide and a 74 amino acid mature chemokine. Mouse CXCL7 shares 72% amino acid sequence identity with rat CXCL7. The recombinant mouse CXCL7 produced by R&D Systems has been shown to chemoattract mouse BaF3 cells transfected with human CXCR2, but not human CXCR1.

Alternate Names

Thymus Chemokine1

Entrez Gene IDs

57349 (Mouse); 246358 (Rat)

Gene Symbol

PPBP

UniProt

AAG36786

Additional CXCL7/Thymus Chemokine-1 Products

Product Documents for Mouse CXCL7/Thymus Chemokine-1 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CXCL7/Thymus Chemokine-1 Biotinylated Antibody

For research use only

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