Mouse CXCL10/IP-10/CRG-2 Alexa Fluor® 594-conjugated Antibody

Name: Mouse CXCL10/IP-10/CRG-2 Alexa Fluor® 594-conjugated Antibody
Brand: R&D Systems
SKU: AF-466-NAT

R&D Systems, part of Bio-Techne | Catalog # AF-466-NAT

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Product Datasheet / COA

Key Product Details

Species Reactivity:	Mouse
Immunogen:	E. coli-derived recombinant mouse CXCL10/IP-10/CRG-2 Ile22-Pro98 Accession # P17515
Applications:	Block/Neutralize, Western Blot
Label:	Alexa Fluor 594 (Excitation=590 nm, Emission=617 nm)
Specificity:	Detects mouse CXCL10/IP-10/CRG-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human CXCL10 is observed.

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Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.

Item	Value
Neutralization	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: CXCL10/IP-10/CRG-2

The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has since been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Recently, human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

Item	Value
Entrez Gene IDs	3627 (Human); 15945 (Mouse)
Alternate Names	C7, CRG-2, CRG2, CXCL10, IFI10, INP10, IP-10, SCYB10, chemokine (C-X-C motif) ligand 10, gIP-10, mob-1

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