Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (Catalog # 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB453R). The ND₅₀ is typically0.05-0.25 µg/mL.

Applications for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CXCL1/GRO alpha/KC/CINC‑1 aa 20-96 (Catalog # 453-KC)

Neutralization

Measured by its ability to neutralize CXCL1/GRO alpha/KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.05-0.25 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha/KC/CINC‑1 aa 20‑96.

Mouse CXCL1/KC Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse CXCL1/GRO alpha/KC/CINC-1 Biotinylated Antibody (Catalog # BAF453)
Standard: Recombinant Mouse CXCL1/KC Protein (Catalog # 453-KC)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1, also known as KC and GRO alpha, is a CXC family chemokine that is expressed in fibroblasts, macrophages, and endothelial cells and interacts with CXCR2. CXCL1 is a potent neutrophil attractant and activator. It also promotes angiogenesis by inducing VEGF production by neutrophils. N-terminally truncated CXCL1 demonstrates increased potency in CXCR2 signaling. Mature mouse CXCL1 shares 96% and 64% aa sequence identity with rat and human CXCL1, respectively.

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha

Entrez Gene IDs

2919 (Human); 14825 (Mouse); 81503 (Rat)

Gene Symbol

CXCL1

UniProt

P12850

Additional CXCL1/GRO alpha/KC/CINC-1 Products

Product Documents for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody

For research use only

Citations
Reviews

Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB453R

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CXCL1/GRO alpha/KC/CINC-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody

Chemotaxis Induced by CXCL1/GRO alpha/KC/CINC-1 and Neutralization by Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody.