Mouse Complement Component C1ra Alexa Fluor™ Plus 488-conjugated Antibody

Name: Mouse Complement Component C1ra Alexa Fluor™ Plus 488-conjugated Antibody
Brand: R&D Systems
SKU: FAB7160AFP488

R&D Systems, part of Bio-Techne | Catalog # FAB7160AFP488

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Product Datasheet / COA / SDS

Key Product Details

Species Reactivity:	Mouse
Label:	Alexa Fluor Plus 488 (Excitation=493 nm, Emission=518 nm)
Specificity:	Detects mouse Complement Component C1ra in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human C1ra is observed.

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Technical Data
Citations / Reviews

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Formulation	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: Complement Component C1ra

C1ra (complement component 1 subcomponent r-A) is an 85-95 kDa member of the peptidase S1 family of enzymes. It is secreted primarily by hepatocytes but is also known to be expressed by monocytes, fibroblasts, keratinocytes and endothelial cells. C1r forms a key component of the complement 1 complex. Complement is a general name for a group of molecules that "complement" the antimicrobial effort associated with antibodies and phagocytic cells. The C1 complex is composed of five proteins; one C1q that binds immunoglobulin, and two copies of two inactive serine proteases termed C1r and C1s. Activation of the C1 complex is the first step in a proteolytic cascade that generates multiple modulators involved in cell lysis and phagocytosis. Following C1q binding, each 85-90 kDa C1r monomer undergoes autocleavage to generate a 60 kDa and 28-34 kDa disulfide‑linked enzymatically-active heterodimer. Each C1r heterodimer now acts on a C1s counterpart to create a globally-active complex that drives the complement cascade. In contrast to human, the mouse genes for C1r and C1s have undergone duplication. The major, or liver derived, C1r is termed C1ra, while a prostate-associated C1r is called C1rB. Mouse C1ra proenzyme is 691 amino acids (aa) in length (aa 17-707). It contains one CUB domain (aa 17-140), an EGF-like motif (aa 141-189), a second CUB domain (aa 192-303), two SUSHI (or CCP) repeats (aa 306-448), and a C-terminal peptidase S1 region (aa 463-704). Autocleavage occurs between Arg462:Ile463, generating a 60 kDa N-terminal subunit that is disulfide‑linked to a 30 kDa enzymatic subunit. Over aa 17-707, mouse C1ra shares 91% and 82% aa sequence identity with rat and human C1r, respectively. Full-length mouse C1ra shares 96% aa sequence identity with mouse C1rB, with virtually all differences arising in the enzymatic, or small subunit.

Item	Value
Entrez Gene IDs	50909 (Mouse)
Alternate Names	C1r;Complement C1r-A subcomponent;mC1;mC1rA;Peptidase S1 domain-containing protein, C1ra, Complement Component C1ra

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Mouse Complement Component C1ra Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7160AFP488

Key Product Details

Applications

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Complement Component C1ra

Customer Reviews

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

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