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Mouse CLEC4F/CLECSF13 Antibody

Catalog # AF2784 | R&D Systems, Inc. a Bio-Techne Brand
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AF2784
AF2784-SP

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse CLEC4F/CLECSF13 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CLEC4F/CLECSF13
Ala65-Gly548
Accession # P70194

Specificity

Detects mouse CLEC4F/CLECSF13 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CLEC4F/CLECSF13 Antibody

CLEC4F/CLECSF13 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr).

CLEC4F/CLECSF13 in Mouse Liver.

CLEC4F/CLECSF13 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse CLEC4F/CLECSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2784) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse CLEC4F/CLECSF13 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CLEC4F/CLECSF13 (Catalog # 2784-CL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using AF2784 in the following applications:

Published Applications

Read 9 publications using AF2784 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC4F/CLECSF13

CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1‑3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N‑acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).

References

  1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  2. Bilzer, M. et al. (2006) Liver Int. 26:1175.
  3. Kuiper, J. et al. (1994) Biochem. J. 299:285.
  4. Accession # P70194.
  5. Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487.
  6. Fadden, A.J. et al. (2003) Glycobiology 13:529.
  7. Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753.
  8. Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433.
  9. Coombs, P.J. et al. (2006) Glycobiology 16:1C.
  10. Biessen, E.A.L. et al. (1994) Biochem. J. 299:291.
  11. Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.

Long Name

C-type Lectin Domain Family 4, Member F

Alternate Names

CLECSF13, Galactose Particle Receptor, KCLR, KCR

Entrez Gene IDs

165530 (Human); 51811 (Mouse); 114598 (Rat)

Gene Symbol

CLEC4F

UniProt

Product Documents for Mouse CLEC4F/CLECSF13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CLEC4F/CLECSF13 Antibody

For research use only

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