Mouse splenocytes were stained with Goat Anti-Mouse CD99 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3905, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (Catalog #NL002).

Applications for Mouse CD99 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample:

Mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD99

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD99

CD99 (also known as PILR-L and MIC2) is a 125 amino acid, 24‑30 kDa glycosylated transmembrane glycoprotein expressed as a homodimer on the surface of endothelial, T and B cells, monocytes and stem cells. CD99 has been implicated in processes like homotypic cell adhesion, diapedesis and oncosupression. The extracellular domain of the mouse CD99 protein used as immunogen is 45% identical to the human counterpart.

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Entrez Gene IDs

4267 (Human); 673094 (Mouse); 652929 (Rat)

Gene Symbol

CD99

UniProt

Q8VCN6

Additional CD99 Products

Product Documents for Mouse CD99 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CD99 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CD99 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3905

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD99 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD99 Antibody

Detection of CD99 in Mouse Splenocytes by Flow Cytometry.