Mouse CD74 APC-conjugated Antibody

Detection of CD74 in A20 Mouse Cell Line by Flow Cytometry.

A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse CD74 APC-conjugated Monoclonal Antibody (Catalog # FAB7478A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of CD74 by Flow Cytometry

Expansion of HSPCs in the BM of CD74−/− mice.(A) BM cells derived from WT or CD74−/− were purified. Histograms show representative analysis of CD74 expression on HSPCs in WT and CD74−/− mice. n = 3. (B) Total BM cellularity per femur and tibia in WT and CD74−/− mice, Data A in S1 Data. (C–J) The percent of the different populations in WT and CD74−/−-derived BM cells. (C) Lin-; Data B in S1 Data(D) Representative FACS analysis of WT and CD74−/− HSPCs; (E) LSK; Data C in S1 Data(F) CD34-/LSK; Data D in S1 Data and (G) CD34+; Data E in S1 Data(H) CD150+CD48-LSK; Data F in S1 Data(I) CD150-CD48-/LSK; Data G in S1 Data and (J) CD150-CD48+/LSK; n = 14–18, Data H in S1 Data. (K) CFUC assay: Total BM cells from WT and CD74−/− mice were seeded at 15,000 cells/mL in semisolid cultures supplemented with cytokines and nutrients. CFU-C were counted 7 days later; n = 7, Data I in S1 Data. Bars show SEM. Unpaired two-tailed t test *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. The fcs files and gates can be found at the Flow Repository (accession number FR-FCM-Z3F2). BM, bone marrow; CFU-C, colony-forming unit cell; FACS, fluorescence-activated cell sorting; HSPC, hematopoietic stem and progenitor cell; WT, wild-type. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33661886), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CD74 by Flow Cytometry

Expansion of HSPCs in the BM of CD74−/− mice.(A) BM cells derived from WT or CD74−/− were purified. Histograms show representative analysis of CD74 expression on HSPCs in WT and CD74−/− mice. n = 3. (B) Total BM cellularity per femur and tibia in WT and CD74−/− mice, Data A in S1 Data. (C–J) The percent of the different populations in WT and CD74−/−-derived BM cells. (C) Lin-; Data B in S1 Data(D) Representative FACS analysis of WT and CD74−/− HSPCs; (E) LSK; Data C in S1 Data(F) CD34-/LSK; Data D in S1 Data and (G) CD34+; Data E in S1 Data(H) CD150+CD48-LSK; Data F in S1 Data(I) CD150-CD48-/LSK; Data G in S1 Data and (J) CD150-CD48+/LSK; n = 14–18, Data H in S1 Data. (K) CFUC assay: Total BM cells from WT and CD74−/− mice were seeded at 15,000 cells/mL in semisolid cultures supplemented with cytokines and nutrients. CFU-C were counted 7 days later; n = 7, Data I in S1 Data. Bars show SEM. Unpaired two-tailed t test *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. The fcs files and gates can be found at the Flow Repository (accession number FR-FCM-Z3F2). BM, bone marrow; CFU-C, colony-forming unit cell; FACS, fluorescence-activated cell sorting; HSPC, hematopoietic stem and progenitor cell; WT, wild-type. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33661886), licensed under a CC-BY license. Not internally tested by R&D Systems.