Mouse CD69 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB2386A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 310106
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD69
Gly64-Arg199
Accession # P37217
Gly64-Arg199
Accession # P37217
Specificity
Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD69 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse CD69 APC-conjugated Antibody
Detection of CD69 in CTLL-2 Mouse Cell Line by Flow Cytometry.
CTLL-2 mouse cytotoxic T cell line was stained with Rat Anti-Mouse CD69 APC-conjugated Monoclonal Antibody (Catalog # FAB2386A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD69 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were (A) cultured with plate-bound anti-CD3 and anti-CD28 antibodies overnight, or (B) unstimulated, then stained with Rat Anti-Mouse CD69 APC-conjugated Monoclonal Antibody (Catalog # FAB2386A) and Rat anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on isotype control antibody (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse CD69 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: CTLL‑2 mouse cytotoxic T cell line, and Anti-CD3/CD28-activated mouse splenocytes
Sample: CTLL‑2 mouse cytotoxic T cell line, and Anti-CD3/CD28-activated mouse splenocytes
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD69
Alternate Names
CD69, EA-1, Leu23, MLR-3, p60
Gene Symbol
CD69
UniProt
Additional CD69 Products
Product Documents for Mouse CD69 APC-conjugated Antibody
Product Specific Notices for Mouse CD69 APC-conjugated Antibody
For research use only
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