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Mouse CD30 Ligand/TNFSF8 Antibody

Bio-Techne includes R&D Systems | Catalog # MAB732

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MAB732-SP
MAB732

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

ELISA Capture (Matched Antibody Pair), Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 82414

Product Summary for Mouse CD30 Ligand/TNFSF8 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD30 Ligand/TNFSF8
Gln68-Asp239
Accession # P32972

Specificity

Detects mouse CD30 Ligand/TNFSF8 in ELISAs and Western blots. In sandwich immunoassays, no cross‑reactivity or interference with recombinant human (rh) CD30, recombinant mouse (rm) CD30, rhCD40 Ligand, rhFas Ligand, rhTNF-alpha, rmTNF-alpha, recombinant rat TNF-alpha, or rhTNF-beta is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse CD30 Ligand/TNFSF8 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CD30 Ligand/TNFSF8 (Catalog # 732-CL)

Mouse CD30 Ligand/TNFSF8 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CD30 Ligand/TNFSF8 Biotinylated Antibody (Catalog # BAF732)
  • Standard: Recombinant Mouse CD30 Ligand/TNFSF8 Protein (Catalog # 732-CL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD30 Ligand/TNFSF8

CD30 Ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells. The specific receptor for CD30L is CD30/TNFRSF8, a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkin's and Reed-Sternberg cells using the monoclonal antibody Ki-1. CD30 is also expressed on different non-Hodgkin's lymphomas, virus-infected T and B cells, and on normal T and B cells after activation. Among T cells, CD30 is preferentially expressed on a subset of T cells producing Th2-type cytokines and on CD4+/CD8+ thymocytes that coexpress CD45RO and IL-4 receptor. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis. CD30 can act as a co‑stimulatory molecule in thymic negative selection and may also play a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas.

References

  1. Brunangelo, F. et al. (1995) Blood 85:1.
  2. Gruss, H-J. and F. Herrmann (1996) Leukemia and Lymphoma 20:397.
  3. Chiarle, R. et al. (1999) J. Immunol. 163:194.

Alternate Names

CD153, CD30L, TNFSF8

Entrez Gene IDs

944 (Human); 21949 (Mouse)

Gene Symbol

TNFSF8

UniProt

Additional CD30 Ligand/TNFSF8 Products

Product Documents for Mouse CD30 Ligand/TNFSF8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD30 Ligand/TNFSF8 Antibody

For research use only

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