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Mouse CD27 Ligand/TNFSF7 Antibody

Catalog # MAB783 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Key Product Details

Species Reactivity



ELISA Capture (Matched Antibody Pair), Neutralization



Antibody Source

Monoclonal Rat IgG1 Clone # 118510

Product Summary for Mouse CD27 Ligand/TNFSF7 Antibody


S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CD27 Ligand/TNFSF7
Accession # O55237


Detects mouse CD27 Ligand/TNFSF7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD30 Ligand or rhCD40 Ligand is observed.







Scientific Data Images for Mouse CD27 Ligand/TNFSF7 Antibody

Cell Proliferation Induced by CD27 Ligand/TNFSF7 and Neutralization by Mouse CD27 Ligand/TNFSF7 Antibody.

Cell Proliferation Induced by CD27 Ligand/TNFSF7 and Neutralization by Mouse CD27 Ligand/TNFSF7 Antibody.

In the presence of sub-optimal amounts of Mouse CD3e Monoclonal Antibody (Catalog # MAB484), Recom-binant Mouse CD27 Ligand/ TNFSF7 (Catalog # 783-CL) stimulates proliferation in mouse splenic T cells in a dose-dependent manner (orange line). Under these conditions, prolif-eration elicited by Recombinant Mouse CD27 Ligand/TNFSF7 (10 µg/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse CD27 Ligand/ TNFSF7 Monoclonal Antibody (Catalog # MAB783). The ND50 is typically 0.5-2 µg/mL.

Applications for Mouse CD27 Ligand/TNFSF7 Antibody

Recommended Usage


Measured by its ability to neutralize CD27 Ligand/TNFSF7-induced proliferation in mouse splenic T cells. The Neutralization Dose (ND50) is typically 0.5-2 µg/mL in the presence of 10 µg/mL Recombinant Mouse CD27 Ligand/TNFSF7 and sub-optimal amounts of Mouse CD3 epsilon Monoclonal Antibody.

Mouse CD27 Ligand/TNFSF7 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: (Catalog # BAF783)
  • Standard: Recombinant Mouse CD27 Ligand/TNFSF7 Protein (Catalog # 783-CL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage


Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.


Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27 Ligand/TNFSF7

CD27 ligand (CD27L), also named CD70, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7 (1, 2). Mouse CD27L cDNA encodes a 195 amino acid (aa) residue protein with a 23 aa N-terminal cytoplasmic domain, a 21 transmembrane domain and a 151 aa C‑terminal extracellular domain. Mouse and human CD27L share approximated 56% aa sequence identity. By analogy to other TNFSF ligands, CD27L is expected to exist as non-covalent homotrimers. The expression of CD27L is highly regulated at the transcription and posttranslational level (3). CD27L cell surface expression is induced by antigen receptor activation in B cells and at low levels in mouse T cells. Although CD27L expression is not detected on human dendritic cells (DC), membrane expression on mature mouse DCs has been reported. CD27L expression is also present in the thymus medulla in both human and mouse. CD27L interacts with CD27, a member of the TNF receptor superfamily that is expressed on natural killer (NK) cells and subsets of T and B cells (2, 4, 5). Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation (2). Ligation of CD27 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses (5, 6). On NK cells, ligation of CD27 induces proliferation and IFN-gamma production (4).


  1. Hitzen, R.Q. et al. (1994) Immunol. Today 15:307.
  2. Croft, M. (2003) Nature Reviews Immunol. 3:609.
  3. Tesselaar, K. et al. (2003) J. Immunol. 169:33.
  4. Takeda, K. et al. (2000) J. Immunol. 164:1741.
  5. Kobata, T.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11249.
  6. Raman, V.S. et al. (2003) J. Immunol. 171:5876.

Alternate Names


Entrez Gene IDs

970 (Human); 21948 (Mouse); 301132 (Rat)

Gene Symbol



Product Documents for Mouse CD27 Ligand/TNFSF7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD27 Ligand/TNFSF7 Antibody

For research use only

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