CD177 is a member of the uPAR/CD59/Ly6 superfamily (1). Mature mouse CD177 is a 796 amino acid (aa) protein that contains four uPAR/Ly6 domains, while human CD177 contains only two. Within common regions, mouse CD177 shares 55% and 77% aa sequence identity with human and rat CD177, respectively. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2-4). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (4, 5). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (6, 7). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).

References

Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
Klippel, S. et al. (2002) Blood 100:2441.
Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
Temerinac, S. et al. (2000) Blood 95:2569.
Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
von Vietinghoff, S. et al. (2007) Blood 109:4487.
Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.

Long Name

Polycythemia Rubra Vera Protein 1

Alternate Names

CD177, HNA-2a, NB1 GP, PRV1

Entrez Gene IDs

57126 (Human); 68891 (Mouse)

Gene Symbol

CD177

UniProt

Q8R2S8

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Product Specific Notices for Mouse CD177 Alexa Fluor™ Plus 405-conjugated Antibody

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Mouse CD177 Alexa Fluor™ Plus 405-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8186AFP405

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Background: CD177