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Mouse CCL9/10/MIP‑1 gamma Antibody

Catalog # MAB463 | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Species Reactivity



ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot



Antibody Source

Monoclonal Rat IgG1 Clone # 62105

Product Summary for Mouse CCL9/10/MIP‑1 gamma Antibody


E. coli-derived recombinant mouse CCL9/10/MIP‑1 gamma
Accession # P51670


Detects mouse CCL9/10/MIP‑1 gamma in ELISAs and Western blots. In sandwich ELISAs, less than 0.02% cross-reactivity with recombinant human CCL3, 4, 15, 19, 20, recombinant mouse CCL3, 4, 19, or recombinant rat CCL20 is observed.







Scientific Data Images for Mouse CCL9/10/MIP‑1 gamma Antibody

Chemotaxis Induced by CCL9/10/MIP‑1 gamma and Neutral-ization by Mouse CCL9/10/ MIP‑1 gamma Antibody.

Chemotaxis Induced by CCL9/10/MIP‑1 gamma and Neutral-ization by Mouse CCL9/10/ MIP‑1 gamma Antibody.

Recombinant Mouse CCL9/10/ MIP-1 gamma (Catalog # 463-MG) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL9/10/MIP-1 gamma (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL9/10/MIP-1 gamma Monoclonal Antibody (Catalog # MAB463). The ND50 is typically 1-4 µg/mL.

Applications for Mouse CCL9/10/MIP‑1 gamma Antibody

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL9/10/MIP‑1 gamma (Catalog # 463-MG) under non-reducing conditions only


Measured by its ability to neutralize CCL9/10/MIP‑1 gamma-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 0.04 µg/mL Recombinant Mouse CCL9/10/MIP‑1 gamma.

Mouse CCL9/10/MIP-1 gamma Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CCL9/10/MIP‑1 gamma Biotinylated Antibody (Catalog # BAF463)
  • Standard: Recombinant Mouse CCL9/10/MIP-1 gamma Protein (Catalog # 463-MG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage


Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.


Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL9/10/MIP-1 gamma

Mouse CCL9/10 (also named MIP-1 gamma and MRP-2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the beta (or CC) intercrine family of chemokines (1‑3). Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six cysteine-containing CC subfamily of chemokines (2, 4, 5). This subfamily contains four N-terminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no human-to-rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded N-terminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines (3‑7). Mouse CCL9/10 is 75% aa identical to rat CCL9/10 (8). Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at full‑length, but are converted to highly active forms upon N-terminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the N-terminus, generating a highly active, 8 kDa, 71‑73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP-1 alpha and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a full‑length molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants (5, 7).


  1. Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
  2. Zlotnik, A. et al. (1999) Crit. Rev. Immunol. 19:1
  3. Mohamadzadeh, M. et al. (1996) J. Immunol. 156:3102.
  4. Haelens, A. et al. (1996) Immunobiology 195:499.
  5. Berahovich, R.D. et al. (2005) J. Immunol. 174:7341.
  6. Youn, B-S. et al. (1995) J. Immunol. 155:2661.
  7. Poltorak, A.N. et al. (1995) J. Inflamm. 45:207.

Alternate Names

CCF18, MIP-1 gamma, MIP1 gamma

Entrez Gene IDs

20308 (Mouse)

Gene Symbol



Product Documents for Mouse CCL9/10/MIP‑1 gamma Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CCL9/10/MIP‑1 gamma Antibody

For research use only

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