Mouse CCL9/10/MIP‑1 gamma Antibody

Name: Mouse CCL9/10/MIP‑1 gamma Antibody
Brand: R&D Systems
SKU: MAB463

Catalog # MAB463 | R&D Systems, Inc. a Bio-Techne Brand

Citations (6)

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG₁ Clone # 62105

Product Summary for Mouse CCL9/10/MIP‑1 gamma Antibody

Immunogen

E. coli-derived recombinant mouse CCL9/10/MIP‑1 gamma
Gln22-Gln122
Accession # P51670

Specificity

Detects mouse CCL9/10/MIP‑1 gamma in ELISAs and Western blots. In sandwich ELISAs, less than 0.02% cross-reactivity with recombinant human CCL3, 4, 15, 19, 20, recombinant mouse CCL3, 4, 19, or recombinant rat CCL20 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Scientific Data Images for Mouse CCL9/10/MIP‑1 gamma Antibody

Chemotaxis Induced by CCL9/10/MIP‑1 gamma and Neutral-ization by Mouse CCL9/10/ MIP‑1 gamma Antibody.

Recombinant Mouse CCL9/10/ MIP-1 gamma (Catalog # 463-MG) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL9/10/MIP-1 gamma (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL9/10/MIP-1 gamma Monoclonal Antibody (Catalog # MAB463). The ND₅₀ is typically 1-4 µg/mL.

Applications for Mouse CCL9/10/MIP‑1 gamma Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL9/10/MIP‑1 gamma (Catalog # 463-MG) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize CCL9/10/MIP‑1 gamma-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND₅₀) is typically 1-4 µg/mL in the presence of 0.04 µg/mL Recombinant Mouse CCL9/10/MIP‑1 gamma.

Mouse CCL9/10/MIP-1 gamma Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse CCL9/10/MIP‑1 gamma Biotinylated Antibody (Catalog # BAF463)
Standard: Recombinant Mouse CCL9/10/MIP-1 gamma Protein (Catalog # 463-MG)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL9/10/MIP-1 gamma

Mouse CCL9/10 (also named MIP-1 gamma and MRP-2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the beta (or CC) intercrine family of chemokines (1‑3). Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six cysteine-containing CC subfamily of chemokines (2, 4, 5). This subfamily contains four N-terminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no human-to-rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded N-terminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines (3‑7). Mouse CCL9/10 is 75% aa identical to rat CCL9/10 (8). Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at full‑length, but are converted to highly active forms upon N-terminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the N-terminus, generating a highly active, 8 kDa, 71‑73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP-1 alpha and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a full‑length molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants (5, 7).

References

Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
Zlotnik, A. et al. (1999) Crit. Rev. Immunol. 19:1
Mohamadzadeh, M. et al. (1996) J. Immunol. 156:3102.
Haelens, A. et al. (1996) Immunobiology 195:499.
Berahovich, R.D. et al. (2005) J. Immunol. 174:7341.
Youn, B-S. et al. (1995) J. Immunol. 155:2661.
Poltorak, A.N. et al. (1995) J. Inflamm. 45:207.

Alternate Names

CCF18, MIP-1 gamma, MIP1 gamma

Entrez Gene IDs

20308 (Mouse)

Gene Symbol

CCL9

UniProt

P51670

Product Documents for Mouse CCL9/10/MIP‑1 gamma Antibody

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Product Specific Notices for Mouse CCL9/10/MIP‑1 gamma Antibody

For research use only

Citations for Mouse CCL9/10/MIP‑1 gamma Antibody (6)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse CCL9/10/MIP‑1 gamma Antibody include:

Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

C Hemmers et al. (2022-02-10), Chemokine CCL9 Is Upregulated Early in Chronic Kidney Disease and Counteracts Kidney Inflammation and Fibrosis Biomedicines, 2022-010(2).
PMID: 35203629
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

L Li et al. (2020-02-25), Astroglial TLR9 antagonism promotes chemotaxis and alternative activation of macrophages via modulation of astrocyte-derived signals: implications for spinal cord injury J Neuroinflammation, 2020-017(1):73.
PMID: 32098620
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

R Chen et al. (2016-09-20), Novel chemokine-like activities of histones in tumor metastasis Oncotarget, 2016-07(38):61728-61740.
PMID: 27623211
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development

Zhang M et al. (2012-01-25), Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-058(2):169-77.
PMID: 22277799
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Okamatsu Y et al. (2004-08-01), MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival. J. Immunol., 2004-0173(3):2084-90.
PMID: 15265944
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development,ELISpot Development

Mosmann T (2004-06-01), Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions. Eur. J. Immunol., 2004-034(6):1617-26.
PMID: 15162431

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