Detects mouse CCL19/MIP-3 beta in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) TECK, rmTARC, recombinant human MIP-3 beta, rmMIP-1 alpha, rmMIP-1 beta, rm6Ckine, and rmRANTES is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CCL19/MIP‑3 beta Biotinylated Antibody

Mouse CCL19 / MIP-3 beta ELISA Standard Curve

Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3) was serially diluted and captured by Rat Anti-Mouse CCL19/MIP-3 beta Monoclonal Antibody (Catalog # MAB880) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse CCL19/MIP‑3 beta Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3)

Mouse CCL19/MIP-3 beta Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Mouse CCL19/MIP-3 beta Antibody (Catalog # MAB880)
Standard: Recombinant Mouse CCL19/MIP-3 beta Protein (Catalog # 440-M3)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL19/MIP-3 beta

CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta chemokine that binds specifically to the chemokine receptor CCR-7/EBI-1/BLR-2. Mouse MIP-3 beta cDNA encodes a 108 amino acid residue precursor protein with a predicted 25 aa residue signal peptide that is cleaved to form the 83 aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T cell zone. The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. The MIP-3 beta receptor (CCR7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up-regulated in B-cells infected with Epstein-Barr virus and T cells infected with herpes virus 6 or 7.

References

Kim, C.H. et al. (1998) J. Immunol. 160:2418.
Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

Alternate Names

ELC, MIP-3 beta, MIP3 beta

Entrez Gene IDs

6363 (Human); 24047 (Mouse); 362506 (Rat)

Gene Symbol

CCL19

UniProt

Q548P0

Additional CCL19/MIP-3 beta Products

Product Documents for Mouse CCL19/MIP‑3 beta Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CCL19/MIP‑3 beta Biotinylated Antibody

For research use only

Citations
Reviews

Mouse CCL19/MIP‑3 beta Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF880

Key Product Details

Species Reactivity

Validated:

Cited:

Applications