Caspase-11/Caspase-4 was detected in immersion fixed mouse splenocytes treated with LPS using Rat Anti-Mouse Caspase-11/Caspase-4 Monoclonal Antibody (Catalog # MAB86481) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse Caspase-11/Caspase-4 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes treated with LPS

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-11/Caspase-4

Mouse Caspase-11 (commonly referred as Caspase-4) is a member of the cysteine-aspartic acid protease family known as caspases that function in initiating apoptosis or activating proinflammitory cytokines. Mouse Caspase-11 is expressed as a 343 amino acid latent zymogen. The active caspase-11 is cleaved into two subunits, p20 (aa 81-266) and p10 (aa 286-373). Caspase-11 is one of several caspases associated with the inflammasome and plays an important role in the activation of proinflammatory cytokines. Caspase-11 is activated by an autocatalytic mechanism or by cleavage by Caspase-8 in response to ER stress or bacterial infection, and its expression and activation can be induced by exposure to LPS. The active enzyme can cleave and activate Caspase-1 which in turn activates IL-1 beta. Mouse Caspase-11 has a 59 and 89% homology to human and rat Caspase-4, respectively.

Alternate Names

CASP11, Caspl, Ich-3

Entrez Gene IDs

12363 (Mouse); 114555 (Rat)

Gene Symbol

CASP4

UniProt

P70343

Additional Caspase-11/Caspase-4 Products

Product Documents for Mouse Caspase-11/Caspase-4 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse Caspase-11/Caspase-4 Antibody

For research use only

Citations
Reviews

Mouse Caspase-11/Caspase-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB86481

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Caspase-11/Caspase-4 Antibody

Caspase-11/Caspase-4 in Mouse Splenocytes.