Mouse Carbonic Anhydrase XII/CA12 Antibody

Name: Mouse Carbonic Anhydrase XII/CA12 Antibody
Brand: R&D Systems
SKU: AF2345

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2345

Citations (2)

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Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase XII/CA12 Ala25-Ser301 Accession # Q8CI85
Applications:	Immunoprecipitation, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human CA12 is observed and less than 5% cross-reactivity with recombinant human CA1, rhCA2, rhCA3, rhCA8, recombinant mouse (rm) CA4, and rmCA9 is observed.

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Applications for Mouse Carbonic Anhydrase XII/CA12 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse Carbonic Anhydrase XII (Catalog # 2345-CA)
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Mouse Carbonic Anhydrase XII (Catalog # 2345-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Carbonic Anhydrase XII/CA12

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Mouse CA12 is predicted to be a type I membrane enzyme (2, 3). The secreted, purified rmCA12 corresponds to the extracellular domain and has both CA activity and esterase activity. To our knowledge, this is the first description of purified rmCA12 with enzymatic activity.

Item	Value
References	Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899. Okazaki, Y. et al. (2002) Nature 420:563.
Entrez Gene IDs	771 (Human); 76459 (Mouse)
Alternate Names	CA-XII, CA12, Carbonate dehydratase XII, Carbonic Anhydrase XII, EC 4.2.1.1, FLJ20151, HsT18816, Tumor antigen HOM-RCC-3.1.3, carbonic anhydrase 12, carbonic anhydrase XIICAXII, carbonic dehydratase

Citations for Mouse Carbonic Anhydrase XII/CA12 Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse Carbonic Anhydrase XII/CA12 Antibody include:

Species: Rat
Sample Types: Whole Cells
Applications: ICC

Akiba Y et al. (2008), CO2 chemosensing in rat oesophagus. Gut, 57(12):1654-64.
PMID: 18682519
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

Akiba Y et al. (2006), Carbonic anhydrases and mucosal vanilloid receptors help mediate the hyperemic response to luminal CO2 in rat duodenum. Gastroenterology, 131(1):142-52.
PMID: 16831598

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Mouse Carbonic Anhydrase XII/CA12 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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