Detects mouse Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In Western blots, 100% cross‑reactivity with recombinant human (rh) CA12, 10% cross-reactivity with rhCA2, and no cross-reactivity with rhCA1, 3, 5A, 5B, 6, 7, 8, 10, 11, 13, recombinant mouse CA4, 9, or 14 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Applications for Mouse Carbonic Anhydrase XII/CA12 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Carbonic Anhydrase XII (Catalog # 2345-CA)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase XII/CA12

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Mouse CA12 is a type I membrane enzyme with both CA activity and esterase activity (2, 3). The amino acid sequence of mouse CA12 shares 97%, 82%, and 68% identity with that of rat, human/chimpanzee/canine, and chicken.

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
Okazaki, Y. et al. (2002) Nature 420:563.

Alternate Names

CA12

Entrez Gene IDs

771 (Human); 76459 (Mouse)

Gene Symbol

CA12

UniProt

Q8CI85

Additional Carbonic Anhydrase XII/CA12 Products

Product Documents for Mouse Carbonic Anhydrase XII/CA12 Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Mouse Carbonic Anhydrase XII/CA12 Antibody

For research use only

Citations
Reviews

Mouse Carbonic Anhydrase XII/CA12 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2345

Key Product Details

Species Reactivity

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Antibody Source

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Product Specifications

Immunogen

Specificity