Mouse Amnionless Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7139AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Amnionless
Specificity
Detects mouse Amnionless in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Amnionless is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Amnionless
AMN (Amnionless) is a 45-49 kDa glycoprotein that is expressed by three cell types in mouse; extraembryonic visceral endoderm, proximal tubule renal epithelium, and absorption intestinal epithelium. AMN is required for gastrulation in rodent (but not human), and participates in Vitamin B12 absorption in the gut. In particular, AMN forms a membrane complex with Cubilin, promoting its proper orientation in the membrane and subsequent binding to the Intrinsic factor: VitB12 complex. Mature mouse AMN is a 439 amino acid (aa) type I transmembrane protein. It contains a 343 aa extracellular region (aa 20-362) that possesses one VWFC domain (aa 203‑254), and a 75 aa cytoplasmic tail. In human, AMN may undergo cleavage to generate a 35 kDa soluble fragment. Over aa 20-362, mouse AMN shares 92% and 67% aa sequence identity with rat and human AMN, respectively.
Additional Amnionless Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
Loading...
Loading...
Loading...
Loading...