Detects mouse Aminoacylase/ACY1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human Aminoacylase/ACY1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Aminoacylase/ACY1 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Aminoacylase/ACY1 (Catalog # 2700-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Aminoacylase/ACY1 (Catalog # 2700-ZN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminoacylase/ACY1

The mouse ACY1 gene encodes aminoacylase, a member of the M20 family of metalloproteases (1). ACY1 plays a general role in the cytosolic breakdown of acetylated amino acids generated during protein degradation. It also interacts with sphingosine kinase type 1, which is involved in promoting cell growth and inhibiting apoptosis of tumor cells (2). The full-length protein is expressed and the purified enzyme is active in the assay.

References

Lindner, H.A. et al. (2003) J. Biol. Chem. 278:44496.
Maceyka, M. et al. (2004) FEBS Lett. 568:30.

Alternate Names

ACY1

Entrez Gene IDs

95 (Human); 109652 (Mouse)

Gene Symbol

ACY1

UniProt

NP_079647

Additional Aminoacylase/ACY1 Products

Product Documents for Mouse Aminoacylase/ACY1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Aminoacylase/ACY1 Antibody

For research use only

Citations
Reviews

Mouse Aminoacylase/ACY1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2700

Key Product Details

Species Reactivity

Validated:

Cited:

Applications