Mouse 5'-Nucleotidase/CD73 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB44881
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 496424
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
Trp29-Lys549
Accession # Q61503
Trp29-Lys549
Accession # Q61503
Specificity
Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse 5'-Nucleotidase/CD73 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse 5'-Nucleotidase/CD73 (Catalog # 4488-EN)
Sample: Recombinant Mouse 5'-Nucleotidase/CD73 (Catalog # 4488-EN)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 5'-Nucleotidase/CD73
CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3). The amino acid sequence of mouse CD73 is 94% and 87% identical to that of rat and human CD73, respectively.
References
- Resta, R. et al. (1993) Gene 133:171.
- Resta, R. et al. (1998) Immunol. Rev. 161:95.
- Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.
Alternate Names
5-NT, CD73, E5NT, eNT, NT5E, NTE
Gene Symbol
NT5E
UniProt
Additional 5'-Nucleotidase/CD73 Products
Product Documents for Mouse 5'-Nucleotidase/CD73 Antibody
Product Specific Notices for Mouse 5'-Nucleotidase/CD73 Antibody
For research use only
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