I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.

Name: Moesin Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-90372

The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining RNA interference. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.

Moesin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90372

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AELKTAMSTPHVAEPAENEQDEQDENGAEASADLRADAMAKDRSEEERTTEAEKNERVQKHLKALTSELANARDESKKTANDMIHAENMRLGRDKYKTLRQIRQGNTKQR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Moesin Antibody - BSA Free (NBP1-90372) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Moesin Antibody - BSA Free

Immunohistochemistry-Paraffin: Moesin Antibody [NBP1-90372]

Immunohistochemistry-Paraffin: Moesin Antibody [NBP1-90372] - Staining in human tonsil and skeletal muscle tissues using anti-MSN antibody. Corresponding MSN RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Moesin Antibody [NBP1-90372]

Immunohistochemistry-Paraffin: Moesin Antibody [NBP1-90372] - Staining of human tonsil shows high expression.

Immunohistochemistry-Paraffin: Moesin Antibody [NBP1-90372]

Immunohistochemistry-Paraffin: Moesin Antibody [NBP1-90372] - Staining of human skeletal muscle shows low expression as expected.

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Applications for Moesin Antibody - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Moesin

Moesin (membrane-organizing extension spike protein) has previously been characterized as a possible receptor protein for heparan sulfate and also as a cytoskeletal linker protein that stabilizes cell surface microvilli, filopodia and lamellipodia. Data indicate that moesin is identical to the 77-kDa band that copurifies with ezrin in its isolation from human placenta (1). Members of the ezrin-radixin-moesin (ERM) family of membrane-cytoskeletal linking proteins have NH2- and COOH-terminal domains that associate with the plasma membrane and the actin cytoskeleton, respectively (2). It has been demonstrated that ezrin-radixin-moesin proteins are rapidly inactivated after antigen recognition through a Vav1-Rac1 pathway. The resulting disanchoring of the cortical actin cytoskeleton from the plasma membrane decreased cellular rigidity, leading to more efficient T cell-antigen-presenting cell conjugate formation (3).

Alternate Names

Membrane-organizing extension spike protein, moesin

Gene Symbol

MSN

Additional Moesin Products

Product Documents for Moesin Antibody - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Moesin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Moesin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90372

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

Format

Concentration

View all Moesin Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Description

Scientific Data Images for Moesin Antibody - BSA Free

Immunohistochemistry-Paraffin: Moesin Antibody [NBP1-90372]

Immunohistochemistry-Paraffin: Moesin Antibody [NBP1-90372]

Immunohistochemistry-Paraffin: Moesin Antibody [NBP1-90372]

Western Blot: Moesin Antibody - BSA Free [NBP1-90372]

Western Blot: Moesin Antibody - BSA Free [NBP1-90372]

Western Blot: Moesin Antibody - BSA Free [NBP1-90372]

Applications for Moesin Antibody - BSA Free

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Moesin

Alternate Names

Gene Symbol

Additional Moesin Products

Product Documents for Moesin Antibody - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Moesin Antibody - BSA Free