MMP-12 Antibody - BSA Free

Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225]

Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - DLD1 xenograft, using MMP12 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225]

Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Human lung adenocarcinoma. MMP12 antibody [N3C1], Internal diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: MMP-12 Antibody - BSA Free [NBP1-31225] -

MMP12 expression is essential for DRP1/FOXM1 regulation in HNC cells. (A) The mRNA and protein expression levels of FOXM1 in siDRP1 cells were examined. Quantification of relative FOXM1 expression is shown. (B,C) Western blotting, QPCR and luciferase activity analysis of MMP12 were determined in SAS cells transfected with FOXM1 or vector control in combination with siDRP1 or negative control. Quantification of relative DRP1, Flag‐FOXM1 and MMP12 expressions is shown. (D,E) Western blotting, QPCR and luciferase activity of MMP12 were analyzed in SAS cells transfected with FOXM1 or vector control in combination with Mdivi‐1 treatment. Quantification of relative DRP1, Flag‐FOXM1, and MMP12 expressions is shown. (F) IHC staining patterns of the HNC tumor tissues for DRP1, FOXM1 and MMP12. Scale bar: 100 um. All data presented as mean +/- SD of three independent experiments. Significance calculated using t‐test. * P < 0.05, ** P < 0.01, *** P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35313071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MMP-12 Antibody - BSA Free [NBP1-31225] -

MMP12 is one of the targets of DRP1 in HNC cells. (A) Heat‐map showing relative alteration of target genes belonging to EMT molecules using QPCR array analysis of SAS cell transfected with siDRP1 compared with the negative control. Red: upregulation; green: downregulation. (B) QPCR was analyzed to validate the expressions of target genes from (A). (C) Impact of DRP1 knockdown or Mdivi‐1 on MMP12 protein expression were demonstrated in SAS and HSC‐3 cells. Quantification of relative DRP1 and MMP12 expressions are shown. (D,E) Cell growth and motility were evaluated in DRP1‐depleted cells transfected with MMP12 using MTT and Transwell assays. Quantification of relative Flag‐MMP12 expression is shown. (F) Studies from GEPIA and Oncomine datasets present the increase of MMP12 mRNA in HNC samples. (G) A positive correlation between DRP1 mRNA and MMP12 mRNA was found in the Oncomine cohort (Peng database, n = 41; Estilo database, n = 31). All data are presented as mean +/- SD of three independent experiments. Significance was calculated using t‐test. In (D) (cell growth) and (F) (GEPIA), statistical analyses were performed using one‐way ANOVA followed by Tukey’s multiple comparison’s test and Wilcoxon signed‐rank test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35313071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MMP-12 Antibody - BSA Free [NBP1-31225] -

MMP12 expression is essential for DRP1/FOXM1 regulation in HNC cells. (A) The mRNA and protein expression levels of FOXM1 in siDRP1 cells were examined. Quantification of relative FOXM1 expression is shown. (B,C) Western blotting, QPCR and luciferase activity analysis of MMP12 were determined in SAS cells transfected with FOXM1 or vector control in combination with siDRP1 or negative control. Quantification of relative DRP1, Flag‐FOXM1 and MMP12 expressions is shown. (D,E) Western blotting, QPCR and luciferase activity of MMP12 were analyzed in SAS cells transfected with FOXM1 or vector control in combination with Mdivi‐1 treatment. Quantification of relative DRP1, Flag‐FOXM1, and MMP12 expressions is shown. (F) IHC staining patterns of the HNC tumor tissues for DRP1, FOXM1 and MMP12. Scale bar: 100 um. All data presented as mean +/- SD of three independent experiments. Significance calculated using t‐test. * P < 0.05, ** P < 0.01, *** P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35313071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MMP-12 Antibody - BSA Free [NBP1-31225] -

MMP12 is one of the targets of DRP1 in HNC cells. (A) Heat‐map showing relative alteration of target genes belonging to EMT molecules using QPCR array analysis of SAS cell transfected with siDRP1 compared with the negative control. Red: upregulation; green: downregulation. (B) QPCR was analyzed to validate the expressions of target genes from (A). (C) Impact of DRP1 knockdown or Mdivi‐1 on MMP12 protein expression were demonstrated in SAS and HSC‐3 cells. Quantification of relative DRP1 and MMP12 expressions are shown. (D,E) Cell growth and motility were evaluated in DRP1‐depleted cells transfected with MMP12 using MTT and Transwell assays. Quantification of relative Flag‐MMP12 expression is shown. (F) Studies from GEPIA and Oncomine datasets present the increase of MMP12 mRNA in HNC samples. (G) A positive correlation between DRP1 mRNA and MMP12 mRNA was found in the Oncomine cohort (Peng database, n = 41; Estilo database, n = 31). All data are presented as mean +/- SD of three independent experiments. Significance was calculated using t‐test. In (D) (cell growth) and (F) (GEPIA), statistical analyses were performed using one‐way ANOVA followed by Tukey’s multiple comparison’s test and Wilcoxon signed‐rank test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35313071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.