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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

CoraFluor 1

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP-10 (R&D Systems, Catalog # 910-MP)
Tyr18-Cys476
Accession # P09238

Specificity

Detects human MMP-10 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) MMP‑3 and rhMMP‑7 is observed under non-reducing conditions. Approximately 10% and 2% cross-reactivity with rhMMP-3 and rhMMP-2 is observed under reducing conditions, respectively. Additionally, less than 1% cross-reactivity with rhMMP-1, -8, -9, -12, -13, and recombinant mouse MMP-9 is observed under reducing and non-reducing conditions.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Applications for MMP-10 Antibody [CoraFluor™ 1]

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: MMP-10

Matrix metalloproteinases belong to a family of proteases that are essential for the breakdown of extracellular matrix (ECM), thus they play an important role in apoptosis, and tumor cell growth, invasion, metastasis, as well as in angiogenesis and wound healing. Most of the MMP's contain a common domain structures, which include a signal sequence, a propeptide, a catalytic domain and a hemopexin-like (Hpx) domain. MMP10 belongs to the Stromelysin group of proteases. Stromelysins degrade ECM proteoglycans, laminin, fibronectin and gelatin.

Long Name

Matrix Metalloproteinase 10

Alternate Names

MMP10, Stromelysin 2

Gene Symbol

MMP10

Additional MMP-10 Products

Product Documents for MMP-10 Antibody [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MMP-10 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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