MIST1 Antibody (6E8/A12/C11P1)

Immunohistochemistry: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478] -

RNA expression profiling identifies MIST1 as a DDX56-downstream target in the regulation of the PTEN-AKT axis. (A) A volcano plot constructed using P-values and fold-change to illustrate the differential downstream target genes between DDX56-silenced MHCC97H cells and control cells (|fold change| ≥2, P < 0.05). Red dots represent significantly upregulated genes while blue dots represent significantly downregulated genes. (B) The top 20 differential downstream genes from RNA-seq results were validated by RT-PCR in MHCC97H cells. (C-D) The mRNA and protein levels of MIST1 in both MHCC97H and Huh-7 cells were detected using RT-PCR and western blotting, respectively. (E) IHC staining shows the expression level of MIST1 in the subcutaneous tumor. Scale bars: 250 um (top), 50 um (bottom). (F) Representative images of DDX56 and MIST1 immunohistochemical staining in human HCC tissues (left). Correlation analysis between DDX56 and MIST1 protein expression in HCC tissues (right). Scale bars: 250 um (low magnification), 50 um (high magnification). (G) Effect of MIST1 knockdown on MHCC97H and Huh-7 cell proliferation was determined by colony formation assay. (H) The indicated protein was determined using a western blot analysis of MHCC97H and Huh-7 cells infected with MIST1-silencing lentivirus (shMIST1) or lentivirus control (shCont). (I) Western blot analysis of the PTEN-AKT axis in MIST1-overexpressed HCC cells, showing that PTEN overexpression reversed the effect of MIST1 on the PTEN-AKT axis. Cells were transfected with 3 ug of the indicated plasmids for 72 h. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36168636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.