MIF Antibody (4E4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04319
![Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]](https://resources.bio-techne.com/images/products/MIF-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-04319-img0003.jpg "Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]")
![Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]](https://resources.bio-techne.com/images/products/MIF-Antibody-4E4-Western-Blot-NBP1-04319-img0002.jpg "Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4E4
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human MIF (1-115 aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for MIF Antibody (4E4) - BSA Free
Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]
Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.![Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]](https://resources.bio-techne.com/images/products/MIF-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-04319-img0004.jpg "Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]")
Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]
Immunocytochemistry/Immunofluorescence: MIF Antibody (4E4) [NBP1-04319] - Analysis of MIF in HeLa cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).![Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]](https://resources.bio-techne.com/images/products/MIF-Antibody-4E4-Western-Blot-NBP1-04319-img0001.jpg "Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]")
Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]
Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Jurkat cell lysateLane 2.: THP-1 cell lysateLane 3.: HeLa cell lysateLane 4.: U937 cell lysateApplications for MIF Antibody (4E4) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1:500-1:2000
Application Notes
Use in IHC reported in scientific literature (PMID:34514093).
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MIF
Long Name
Macrophage Migration Inhibitory Factor
Alternate Names
EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase
Gene Symbol
MIF
UniProt
Additional MIF Products
Product Documents for MIF Antibody (4E4) - BSA Free
Product Specific Notices for MIF Antibody (4E4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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