MIER1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35857
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 110-160 of human MIER1 (Q8N108).
Sequence:
SGENKEENIKDSSGQEDETQSSNDDPSQSVASQDAQEIIRPRRCKYFDTNS
Sequence:
SGENKEENIKDSSGQEDETQSSNDDPSQSVASQDAQEIIRPRRCKYFDTNS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit MIER1 Antibody - BSA Free (NBP3-35857) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MIER1 Antibody - BSA Free
Immunohistochemistry: MIER1 Antibody [NBP3-35857] -
Immunohistochemistry: MIER1 Antibody [NBP3-35857] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MIER1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Western Blot: MIER1 Antibody [NBP3-35857] -
Western Blot: MIER1 Antibody [NBP3-35857] - Western blot analysis of various lysates using MIER1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Applications for MIER1 Antibody - BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MIER1
Alternate Names
DKFZp781G0451, Early response 1, Er1, hMi-er1, KIAA1610MI-ER1, mesoderm induction early response 1 (MI-ER1), mesoderm induction early response 1 homolog (Xenopus laevis), mesoderm induction early response protein 1, MGC131940, MGC150640, MGC150641, Mi-er1
Gene Symbol
MIER1
Additional MIER1 Products
Product Documents for MIER1 Antibody - BSA Free
Product Specific Notices for MIER1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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