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MHC Class II Antibody (M5/114) - Azide and BSA Free

Catalog # NBP2-21789 | Novus Biologicals a Bio-Techne Brand

Key Product Details

Species Reactivity

Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # M5/114

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for MHC Class II Antibody (M5/114) - Azide and BSA Free

Immunogen

Activated C57BL/6 mouse spleen cells

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34166814).

Specificity

The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for MHC Class II Antibody (M5/114) - Azide and BSA Free

Flow Cytometry: MHC Class II Antibody (M5/114) - Azide Free [NBP2-21789] - Staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.

Applications for MHC Class II Antibody (M5/114) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

2 ug/ml

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Frozen

1:50-1:300

Immunohistochemistry-Paraffin

1:50-1:300

Immunoprecipitation

1:10-1:500

Western Blot

1:500-1:2000
Application Notes
Functional Application: Blocking of T cell proliferative responses. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using NBP2-21789 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MHC Class II

MHC(major histocompatibility complex)class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

Alternate Names

class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM

Gene Symbol

HLA-DMA

Product Documents for MHC Class II Antibody (M5/114) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MHC Class II Antibody (M5/114) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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