METTL14 Antibody (CL4254) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59044
![Western Blot: METTL14 Antibody (CL4254) [NBP2-59044] Western Blot: METTL14 Antibody (CL4254) [NBP2-59044]](https://resources.bio-techne.com/images/products/METTL14-Antibody-CL4254-Western-Blot-NBP2-59044-img0006.jpg)
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL4254
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q9HCE5, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for METTL14 Antibody (CL4254) - BSA Free
Western Blot: METTL14 Antibody (CL4254) [NBP2-59044]
Western Blot: METTL14 Antibody (CL4254) [NBP2-59044] - Analysis in human cell line A-431.Immunocytochemistry/ Immunofluorescence: METTL14 Antibody (CL4254) [NBP2-59044]
Immunocytochemistry/Immunofluorescence: METTL14 Antibody (CL4254) [NBP2-59044] - Staining of RH-30 cells using the Anti-METTL14 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).Immunohistochemistry-Paraffin: METTL14 Antibody (CL4254) [NBP2-59044]
Immunohistochemistry-Paraffin: METTL14 Antibody (CL4254) [NBP2-59044] - Staining of human duodenum shows strong nuclear immunoreactivity in glandular cells and in the connective tissue.Applications for METTL14 Antibody (CL4254) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: METTL14
Long Name
Methyltransferase-Like Protein 14
Alternate Names
KIAA1627
Gene Symbol
METTL14
Additional METTL14 Products
Product Documents for METTL14 Antibody (CL4254) - BSA Free
Product Specific Notices for METTL14 Antibody (CL4254) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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